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Application of CYP1B1rs162549 in preparing reagent for predicting biochemical reoccurrence risk of prostatic cancer after radical operation

A 1. CYP1B1, prostate cancer technology, applied in the field of molecular biology, can solve the problems of high postoperative recurrence rate and no prostate cancer

Inactive Publication Date: 2018-07-24
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Radical prostatectomy is currently the standard treatment for clinically localized prostate cancer, but the recurrence rate after surgery is as high as 30% within 10 years
There is no study on the association between CYP1B1 gene polymorphisms and recurrence after radical prostatectomy

Method used

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  • Application of CYP1B1rs162549 in preparing reagent for predicting biochemical reoccurrence risk of prostatic cancer after radical operation
  • Application of CYP1B1rs162549 in preparing reagent for predicting biochemical reoccurrence risk of prostatic cancer after radical operation
  • Application of CYP1B1rs162549 in preparing reagent for predicting biochemical reoccurrence risk of prostatic cancer after radical operation

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Embodiment 1

[0017] 1. Research object

[0018] A total of 426 patients with localized prostate cancer who underwent radical prostatectomy in Fudan University Cancer Hospital from 2006 to 2009 were included. The median follow-up time was 37.7 months, and the changes in PSA were followed up every 3 months after surgery. Two consecutive serum PSA levels ≥ 0.2ng / ml were defined as biochemical recurrence. Clinicopathological data and follow-up information were retrieved through the electronic medical record system. Patients who received adjuvant endocrine or radiotherapy postoperatively were excluded.

[0019] 2. Candidate SNP selection and genotyping detection

[0020] Use the human genome database NCBI to find the CYP1B1 gene and determine the research area. The genotyping data information of SNP sites in the study area was searched through the HapMap database, and the genotyping data of all SNP sites in the CYP1B1 gene and its extended regions in the Han population were obtained. Import...

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Abstract

The invention discloses application of CYP1B1 rs162549 in preparing a reagent for predicting the biochemical reoccurrence risk of prostatic cancer after the radical operation, and particularly relatesto application of a primer or probe for detecting a polymorphism variation site of a CYP1B1 gene in preparing a reagent for predicting the biochemical reoccurrence risk of the prostatic cancer afterthe radical operation. A kit for predicting the biochemical reoccurrence risk of the prostatic cancer after the radical operation comprises a primer and probe for detecting a genotype of a polymorphism variation site rs16254 of a CYP1B1 gene. The CYP1B1 SNP rs162549 is related to the biochemical reoccurrence risk of the prostatic cancer after the radical operation. The reoccurrence risk of a carrier of the site TT genotype is significantly higher than that of other genotypes. The genetic typing of the site for the patient after the radical operation of the prostatic cancer is favorable for determining the reoccurrence risk, so that the adjuvant therapy is adopted for the high-risk patient to improve the prognosis.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to the application of CYP1B1rs162549 in the preparation of reagents for predicting the risk of biochemical recurrence after radical prostatectomy. Background technique [0002] Radical prostatectomy is currently the standard treatment for clinically localized prostate cancer, but the postoperative recurrence rate is as high as 30% within 10 years. Prostate specific antigen (PSA) and pathological indicators have low specificity and sensitivity in predicting postoperative recurrence. There is an urgent need for clinical biomarkers that can predict postoperative recurrence. Ideal biomarkers will help Precise adjuvant therapy for patient recurrence risk stratification can improve prognosis. [0003] Previous studies have shown that estrogen plays an important role in the progression of prostate cancer through oxidative metabolism. CYP1B1 is a key enzyme in the hydroxylation process of es...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 顾成元叶定伟戴波朱耀秦晓健吴俊龙
Owner FUDAN UNIV SHANGHAI CANCER CENT