Solid microbial agent and preparation method thereof
A microbial agent and solid adsorbent technology, applied in the field of solid microbial agent and its preparation, can solve the problems of bacterial loss, no sustained release effect, microbial death, etc., and achieve the effect of reducing inactivation
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Embodiment 1
[0026] Add 28g of PEG (Mn=1000) and 12g of PPG (Mn=700) into 100g of diglyme, stir well to dissolve them, and obtain a coating agent solution.
[0027] Add 200 g of diatomaceous earth (d=150-180 μm) to 100 g of Bacillus subtilis fermentation liquid, stir well and absorb to obtain a slurry-like solid adsorbent loaded with microorganisms.
[0028] The solid adsorbent loaded with microorganisms is added to the aforementioned coating agent solution, fully stirred to make the film fully formed, and then spray-dried at 65° C. to obtain a solid microbial bacterial agent.
Embodiment 2
[0030] 60g of poly(ethylene glycol-propylene glycol-ethylene glycol) block copolymer (ethylene glycol segment accounts for 50wt.%, Mn=1800) is dropped into 200g of diglyme, fully stirred to dissolve it, and obtain Coating agent solution.
[0031] 50g Bacillus megaterium (Bacillus megaterium) fermented liquid, 30g jelly-like bacillus (Bacillus mucilagimosuskrassilm) fermented liquid and 20g bacillus amyloliquefaciens (bacillus amgloliquefaciens) fermented liquid were mixed with 300g solid adsorbent (diatomaceous earth: kaolin: zeolite powder=10 :10:80, weight ratio) mixed, fully stirred, and adsorbed to obtain a slurry-like solid adsorbent loaded with microorganisms.
[0032] The solid adsorbent loaded with microorganisms is added to the aforementioned coating agent solution, fully stirred to make the film fully formed, and then spray-dried at 75°C to obtain a solid microbial bacterial agent.
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