Solid microbial agent and preparation method thereof

A microbial agent and solid adsorbent technology, applied in the field of solid microbial agent and its preparation, can solve the problems of bacterial loss, no sustained release effect, microbial death, etc., and achieve the effect of reducing inactivation

Pending Publication Date: 2018-07-27
武汉天度植物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned technical solution is simply to load the liquid preparation to obtain a solid preparation. This kind of preparation, especially in the field of agricultural chemistry, once put into the soil, will cause the bacterial species to be quickly lost with the rain, without any slow-release effect.
Also disclosed in the prior art is coated granular microbial agent (CN102925426A), but its purpose is to prevent the adhesion between particles, and it adopts such as molten urea, water glass, etc. as coating materials, objectively there are microorganisms The problem of death, and this coating material is still unable to achieve the sustained release of microbial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 28g of PEG (Mn=1000) and 12g of PPG (Mn=700) into 100g of diglyme, stir well to dissolve them, and obtain a coating agent solution.

[0027] Add 200 g of diatomaceous earth (d=150-180 μm) to 100 g of Bacillus subtilis fermentation liquid, stir well and absorb to obtain a slurry-like solid adsorbent loaded with microorganisms.

[0028] The solid adsorbent loaded with microorganisms is added to the aforementioned coating agent solution, fully stirred to make the film fully formed, and then spray-dried at 65° C. to obtain a solid microbial bacterial agent.

Embodiment 2

[0030] 60g of poly(ethylene glycol-propylene glycol-ethylene glycol) block copolymer (ethylene glycol segment accounts for 50wt.%, Mn=1800) is dropped into 200g of diglyme, fully stirred to dissolve it, and obtain Coating agent solution.

[0031] 50g Bacillus megaterium (Bacillus megaterium) fermented liquid, 30g jelly-like bacillus (Bacillus mucilagimosuskrassilm) fermented liquid and 20g bacillus amyloliquefaciens (bacillus amgloliquefaciens) fermented liquid were mixed with 300g solid adsorbent (diatomaceous earth: kaolin: zeolite powder=10 :10:80, weight ratio) mixed, fully stirred, and adsorbed to obtain a slurry-like solid adsorbent loaded with microorganisms.

[0032] The solid adsorbent loaded with microorganisms is added to the aforementioned coating agent solution, fully stirred to make the film fully formed, and then spray-dried at 75°C to obtain a solid microbial bacterial agent.

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Abstract

The invention belongs to the field of agricultural biochemical products, and particularly relates to a solid microbial agent and a preparation method thereof. The solid microbial agent is prepared from the following raw materials in parts by weight: 100 parts of microbial fermentation solution, 150 to 300 parts of absorbent and 40 to 60 parts of coating agent; the coating agent is a mixture of polyethylene glycol and polypropylene glycol according to a weight ratio of (60: 40) to (80:20) or a poly(ethylene glycol-propylene glycol)copolymer with the content of polyethylene glycol being 60 to 80percent; and the coating agent is dissolved in the diol ether solvent to form a film. The microbial agent is provided in a solid form, thereby being convenient to transport, store and use; and meanwhile, the solid microbial agent has the expected sustained-release performance. The invention further relates to a preparation method of the solid microbial agent.

Description

technical field [0001] The invention belongs to the field of agricultural biochemical products, and in particular relates to a solid microbial agent and a preparation method thereof. Background technique [0002] Microbial agent refers to the product that is considered to have a large number of beneficial microorganisms with specific functions after they are produced and multiplied, and then made into a product containing specific substances in the living bacteria. Show), not only in the field of pollutant treatment, but also in many agricultural fields such as increasing fertilizer and production, sterilization and sterilization ("Revised Edition of Manual of Common Fertilizers", Tu Shihua). [0003] In the prior art, the application of microbial agents focuses on strain selection, compounding, and fermentation production (CN107058167A, CN105400720A, CN107118998A), and its products are generally bacteria-containing fermentation liquids, so there are difficulties in product ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N11/14C12R1/125C12R1/11C12R1/07C12R1/10
CPCC12N1/16C12N1/20C12N11/14
Inventor 吴鸿翔
Owner 武汉天度植物科技有限公司
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