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Multi-functional tumor-targeted nanometer preparation and construction method and application in treating tumors thereof

A nano-preparation and tumor-targeting technology, applied in the field of biomedicine, can solve the problems of incomplete inhibition of tumor growth and large side effects, and achieve the effects of easy operation, low cost and simple process

Inactive Publication Date: 2018-07-31
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, photothermal therapy cannot completely inhibit the growth of tumors and achieve the effect of reducing or eliminating tumor cells in patients with related cancers.
[0005] Traditional tumor treatment methods have large side effects, and in recent years, emerging tumor treatment methods have certain defects, and the perfect means of tumor treatment needs to be established urgently.

Method used

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  • Multi-functional tumor-targeted nanometer preparation and construction method and application in treating tumors thereof
  • Multi-functional tumor-targeted nanometer preparation and construction method and application in treating tumors thereof
  • Multi-functional tumor-targeted nanometer preparation and construction method and application in treating tumors thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Synthesis and use of multifunctional nano-photothermal preparations

[0033] 1. Synthesis of multifunctional tumor-targeted FA-GNR:

[0034] (1) Chemically synthesized GNR: Gold nanorod GNR was synthesized according to the chemical reduction method, and determined by TEM characterization. The results are as follows figure 1 As shown in A;

[0035] (2) Chemical synthesis of MUA-PEI / MUA-PEI-FA: According to the condensation acylation reaction, in HCl 3 Prepare MUA-PEI in a solvent; prepare MUA-PEI-FA in a DMSO solvent;

[0036] (3) Chemical synthesis of FA-GNR: According to the bonding between -SH and Au, the surface of GNR was modified with MUA-PEI and MUA-PEI-FA in a molar ratio of 1:1, and FA-GNR was synthesized. Characterized by Fourier transform infrared spectroscopy, the results are as follows figure 1 Shown in B and 1C.

[0037] 2. Synthesis of FA-GNR-siRNA carrying siRNA for synergistic treatment of tumors:

[0038] According to the electrostatic ...

Embodiment 2

[0041] Embodiment 2: FA-GNR-siIDO intravenous administration treats mouse LLC lung cancer model

[0042] Targeting mouse LLC cells in vivo by intravenous administration ( image 3 (a)), silencing the tumor-associated target gene IDO( image 3 (b) and 3(c)), improve anti-tumor immune response in vivo, increase tumor local lymphocyte infiltration, anti-tumor immune-related molecules TNF-α and IFN-γ secretion, and reduce CTL cell apoptosis ( Figure 4 ), and under the induction of 808nm infrared laser, generate heat to synergistically kill tumor cells and inhibit the growth of lung cancer ( Figure 5 ).

Embodiment 3

[0043] Embodiment 3: FA-GNR-siBRAF local skin administration treats mouse melanoma model

[0044] In vivo targeting of mouse B16-BL6 cells by topical skin administration ( Figure 6 (D)), silencing the tumor-associated target gene BRAF ( Figure 6 E and 6F), blocking tumor cell BRAF-MEK-ERK pathway ( Figure 7 A), reduce tumor cell invasion, migration and growth ( Figure 7 B and 7C), and induce tumor cell apoptosis ( Figure 8 A). And under the induction of 808nm infrared laser, heat is generated to kill tumor cells synergistically and inhibit the growth of melanoma ( Figure 8 B and 8C).

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Abstract

The invention provides a multi-functional tumor-targeted nanometer preparation and a construction method and application in treating tumors thereof. By means of the nanometer preparation, the tumors can be treated by using the RNA interference technology cooperating with the photothermal therapy, the RNA interference technology and the photothermal therapy cooperatively inhabit the growth of the tumors, and a better tumor treatment effect is achieved. The nanometer preparation comprises effector molecules, a nanometer material and targeting molecules, wherein the effector molecules comprise nucleic acid molecules for RNA interference; a photothermal effect is formed by the nanometer material; the targeting molecules are used for delivering the particular cells which the nanometer preparation arrives at. The nanometer preparation process is mild in condition, the cost is low, and the nanometer preparation is good in biocompatibility, targeting ability, siRNA loading and unloading capacity, serum protection effect and tumor-suppression effect. The industrialization implementating prospect of the nanometer preparation is wide, and the corresponding treatment method is a new potentialtumor treatment thought.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a novel multifunctional nano-photothermal preparation capable of targeted delivery of effector molecules to tumors, its construction method and its application in tumor treatment. Background technique [0002] Tumor has always been a difficult problem for the medical field to overcome. The occurrence of tumors involves a variety of molecular and genetic changes, including the mutation and amplification of proto-oncogenes or the loss and inactivation of tumor suppressor genes. Traditional tumor treatment methods mainly include surgery, chemotherapy, radiotherapy and interventional therapy. At this stage, a combination of surgery, chemotherapy, and radiotherapy is generally used to prevent tumor cell metastasis, dissemination, and planting. However, surgery can only remove tumors that are visible to the naked eye, and cannot remove invisible subclinical lesions and tumor cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/7088A61K47/54A61P35/00
CPCA61K41/0052A61K31/7088A61K47/545A61P35/00A61K2300/00
Inventor 张瑜娟闵卫平袁铿战雪林冯媛媛宋娜
Owner NANCHANG UNIV
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