Micro-extraction method based on phase transition properties of temperature-sensitive polymers

Abstract

The invention provides a micro-extraction method based on phase transition properties of temperature-sensitive polymers. The method comprises the steps of sequentially adding an aqueous solution containing organic pollutant and the temperature-sensitive polymers to a comparison tube; placing the comparison tube in a water bath pot at 40 DEG C, after the aqueous solution is changed from clarification to turbidity, adding inorganic salt, and centrifuging and separating solid phase materials; adding a small amount of organic solvent to the collected solid phase materials for extraction. The micro-extraction method based on the phase transition properties of the temperature-sensitive polymers can extract the organic pollutant with a small amount of organic solvent, has high extraction rate, and is simple and quick to operate.

Description

technical field

[0001] The invention relates to a micro-extraction method, in particular to a micro-extraction method based on the phase transition properties of temperature-sensitive polymers. Background technique

[0002] With the rapid development of modern industrial and agricultural production, associated organic pollutants such as pesticides, chemical fertilizers and industrial wastes (such as PAHs, PCBs, aromatic nitro compounds, etc.) continue to enter the environment, pollute the environment, destroy the ecological balance, and also pass through the food chain Ways to endanger human health. In view of its harmfulness, researchers have carried out a lot of detection and analysis research work on it. Since the content of these organic pollutants in the environment is very low, they need to be analyzed after pre-treatment and enrichment. The commonly used pretreatment methods are liquid-liquid microextraction and solid-phase microextraction.

[0003] Single-drop mic...

Examples

Embodiment

[0020] A kind of micro-extraction method based on temperature-sensitive polymer phase transition property, comprises the steps:

[0021] 1. Add 20mL of water sample containing dibutyl phthalate and 10mg of β-CD-PNIPAM to the colorimetric tube to form a mixed aqueous solution; the concentration of dibutyl phthalate in the water sample is 1ug / mL , the concentration of β-CD-PNIPAM in the mixed aqueous solution is greater than the concentration of dibutyl phthalate;

[0022] 2. Put the colorimetric tube into a 40°C water bath. Since β-CD-PNIPAM is a temperature-sensitive polymer, the solution turns from clear to cloudy after heating. Add 2g of sodium sulfate to make the cloudy liquid form a solid-phase substance and a liquid Two phases, the solid phase material is collected by centrifugation, and the solid phase material is the solid phase material formed after the polymer β-CD-PNIPAM absorbs the organic pollutant dibutyl phthalate.

[0023] 3. Add 200 uL of ethyl acetate to the ...

technical field

[0001] The invention relates to a micro-extraction method, in particular to a micro-extraction method based on the phase transition properties of temperature-sensitive polymers. Background technique

[0002] With the rapid development of modern industrial and agricultural production, associated organic pollutants such as pesticides, chemical fertilizers and industrial wastes (such as PAHs, PCBs, aromatic nitro compounds, etc.) continue to enter the environment, pollute the environment, destroy the ecological balance, and also pass through the food chain Ways to endanger human health. In view of its harmfulness, researchers have carried out a lot of detection and analysis research work on it. Since the content of these organic pollutants in the environment is very low, they need to be analyzed after pre-treatment and enrichment. The commonly used pretreatment methods are liquid-liquid microextraction and solid-phase microextraction.

[0003] Single-drop mic...

Examples

Embodiment

[0020] A kind of micro-extraction method based on temperature-sensitive polymer phase transition property, comprises the steps:

[0021] 1. Add 20mL of water sample containing dibutyl phthalate and 10mg of β-CD-PNIPAM to the colorimetric tube to form a mixed aqueous solution; the concentration of dibutyl phthalate in the water sample is 1ug / mL , the concentration of β-CD-PNIPAM in the mixed aqueous solution is greater than the concentration of dibutyl phthalate;

[0022] 2. Put the colorimetric tube into a 40°C water bath. Since β-CD-PNIPAM is a temperature-sensitive polymer, the solution turns from clear to cloudy after heating. Add 2g of sodium sulfate to make the cloudy liquid form a solid-phase substance and a liquid Two phases, the solid phase material is collected by centrifugation, and the solid phase material is the solid phase material formed after the polymer β-CD-PNIPAM absorbs the organic pollutant dibutyl phthalate.

[0023] 3. Add 200 uL of ethyl acetate to the ...

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