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Isotopic methods for measurement of tryptophan and metabolites thereof

A technology of tryptophan, metabolites, applied in the direction of measuring devices, labels used in chemical analysis, biological tests, etc.

Active Publication Date: 2018-07-31
ELECTROPHORETICS LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] While inhibition of tryptophan degradation has been studied as a means of suppressing immune evasion in cancer, induction of tryptophan degradation has been implicated in the control of autoimmune disease (6), excessive inflammatory disease tolerance (7), chronic infection and allergy

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  • Isotopic methods for measurement of tryptophan and metabolites thereof
  • Isotopic methods for measurement of tryptophan and metabolites thereof
  • Isotopic methods for measurement of tryptophan and metabolites thereof

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Embodiment

[0149] Certain aspects and embodiments of the invention will now be illustrated by way of example and with reference to the above figures and tables. All documents mentioned in this specification are hereby incorporated by reference in their entirety for all purposes.

[0150] Materials and methods

[0151] Cell Cultures and Reagents

[0152] In supplemented with the indicated L-tryptophan (Sigma-Aldrich, Taufkirchen, Germany), 300 mg / l glutamine (Carl Roth, Karlsruhe, Germany), 10% FBS (Thermo Fisher Scientific Inc., Waltham, MA, USA ) and 100 U / mL penicillin and 100 μg / mL streptomycin (PAA Laboratories, Pasching, Australia) in McCoy's 5A medium (BioConcept, Allschwil, Switzerland) to culture SKOV-3 ovarian cancer cells. Human embryonic kidney (HEK) cells were cultured in DMEM (PAA Laboratories), 10% FBS (Thermo Scientific), and 100 U / mL penicillin and 100 μg / mL streptomycin (PAA Laboratories). Peripheral blood mononuclear cells (PBMC) were isolated from healthy non-rela...

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Abstract

Tryptophan degradation is a key metabolic pathway controlling immune reactions and evidence suggests that during cancer progression generation of tryptophan metabolites may be fundamental for immune escape promoting the malignant phenotype of cancer cells in an autocrine fashion. The invention relates to methods of measuring mass tag labelled tryptophan and metabolites thereof and methods using the labelled molecules for monitoring in a subject the effectiveness of a treatment and of disease recurrence after treatment, for stratifying patients and for diagnosing suppression of an immune response in a subject.

Description

technical field [0001] The present invention relates to methods of measuring mass tagged tryptophan and its metabolites, and the use of labeled molecules in subjects to monitor the effectiveness of treatment and disease relapse after treatment, for stratifying patients and diagnosing subjects Methods of Suppression of Immune Responses. Background technique [0002] The recent definition of "evading immune destruction" as a new hallmark of cancer reflects a growing recognition of immune suppression and evasion as key features of malignancy (1). Over the past few years, tryptophan degradation has emerged as a key metabolic pathway controlling immune responses. Emerging evidence suggests that during cancer progression, tryptophan catabolic enzymes indoleamine-2,3-dioxygenase (IDO) and / or tryptophan-2,3-dioxygenase ( Kynurenine production by TDO) may represent a central pathway of immune evasion, while also promoting the malignant phenotype of cancer cells in an autocrine mann...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6848G01N33/532G01N2458/15
Inventor S·康卡雷维奇K·库恩P·舒尔茨-克纳佩I·H·派克C·奥皮茨M·普拉滕
Owner ELECTROPHORETICS LTD
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