A dual-capture nucleic acid extraction microfluidic chip

Abstract

The invention discloses a double-capture nucleic acid extraction microfluidic chip, which belongs to the technical field of nucleic acid extraction; the technical problem to be solved is to provide a double-capture nucleic acid extraction microfluidic chip with simple operation, high detection accuracy and simple manufacturing process ; The technical solution adopted to solve this technical problem is: comprising an inlet unit, a nucleic acid extraction unit and an outlet unit, the inlet unit is connected to the nucleic acid extraction unit through a microfluidic channel, the nucleic acid extraction unit is connected to the outlet unit through a microfluidic channel, and the inlet unit includes a microfluidic channel. Bead solution inlet, cell solution inlet, cell lysate inlet, high-salt solution inlet and low-salt eluent inlet, each solution inlet is provided with a micro-valve on the micro-flow pipeline connected to the rear, and the nucleic acid extraction unit includes a cell capture array and a micro- In the bead capture array, the outlet unit includes a waste liquid outlet and a nucleic acid solution outlet, and a microvalve is arranged on a microfluidic pipeline connected in front of each solution outlet; the invention can be widely used in the field of nucleic acid extraction.

Description

technical field [0001] The invention discloses a double-capture nucleic acid extraction microfluidic chip, which belongs to the technical field of nucleic acid extraction. Background technique [0002] In recent years, nucleic acid molecular diagnostic methods have become a hot spot in biology and medical research, and various new methods and technologies have also emerged. Polymerase chain reaction (polymerase chain reaction, PCR) technology is one of them. Since 1985, Mullis of the American PE company invented the PCR technology, and published the first academic paper on the PCR technology in the journal Science. PCR technology is widely used in the detection of pathogens, genetic abnormalities, tumor diagnosis and monitoring, disease susceptibility detection, food monitoring, veterinary disease diagnosis, water quality monitoring, etc. It has been widely used in fields such as forensic science and forensic science. [0003] Nucleic acid extraction is a prerequisite for...

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Problems solved by technology

However, when the magnetic nanoparticles are separated from the nucleic acid, they are easily mixed in the eluent for subsequent nucleic acid amplification, which will inevitably bring interference to the nucleic acid amplification.

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