Tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation and preparation process thereof

A technology of streptococcal disease and Chinese herbal medicine, which is applied in the field of anti-tilapia streptococcal Chinese herbal medicine preparation and its preparation technology, which can solve the problems of excessive drug residue, drug resistance of bacteria, and poor control effect, so as to improve the efficacy of the drug Effect

Inactive Publication Date: 2018-08-21
GUANGXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional treatment method is to add antibiotics to the feed. Long-term use of such drugs will not only pollute the ecological environment, but also lead to drug-induced diseases and drug residues, making pathogenic bacteria resistant to drugs, and the control effect is not good. Aquaculture costs, and cause drug residues to exceed the standard

Method used

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  • Tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation and preparation process thereof
  • Tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation and preparation process thereof
  • Tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation and preparation process thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The optimization of extraction process conditions for anti-tilapia streptococcosis Chinese herbal medicine preparations is based on the multiples of water addition, water bath time, water bath times, and alcohol precipitation concentration as the main factors under the condition that the medicinal material batches, concentration, and filtration conditions are consistent. determined the investigation level table, and used the orthogonal experiment design method to design the extraction conditions of anti-tilapia streptococcosis Chinese herbal medicine preparations, and determined the contents of baicalin, forsythin, emodin and The yield of the extract was analyzed by variance analysis. Taking baicalin, forsythin, and emodin as indicators, the orthogonal experiment design is shown in Table 1.

[0022] Table 1 Orthogonal factor level L 9 (3 4 )surface

[0023]

[0024] Table 2 Sanhuanglian mixture extraction process orthogonal test analysis results

[0025]

[00...

Embodiment 2

[0028] Specificity test Accurately weigh 30 mg of baicalin reference substance in a 10 mL volumetric bottle and dilute to the mark with methanol, shake well, and use it as a stock solution (3 mg / mL), then accurately pipette 2.5 mL of the stock solution into a 10 mL volumetric bottle and use Dilute methanol to the mark and shake well to obtain the baicalin reference solution (750 μg / mL). Accurately weigh 24mg of forsythin reference substance in a 10mL volumetric bottle and dilute to the mark with methanol, shake well, and use it as a stock solution (2.4mg / mL), then accurately pipette 6.25mL of the stock solution into a 100mL volumetric bottle and mix with methanol Dilute to the mark and shake well to obtain the forsythin reference solution (150 μg / mL). Accurately weigh 10 mg of emodin reference substance in a 10 mL volumetric bottle and dilute to the mark with methanol, shake well, and use it as a stock solution (1 mg / mL), then accurately pipette 1 mL of the stock solution into...

Embodiment 3

[0031] Precision test Precisely pipette the baicalin and forsythin reference substance solutions under the item 2 of Example 2 and dilute them with methanol to a solution of 600 μg / mL each, and precisely pipette the emodin reference substance solution to prepare a 300 μg / mL solution. Mix in equal amounts to prepare a mixed reference solution containing baicalin 200 μg / mL, forsythin 200 μg / mL, and emodin 100 μg / mL. The mixed reference substance solution was injected into the liquid chromatograph with 10 μ L, and the chromatographic conditions described in Example 2 were continuously injected 6 times to measure the peak areas of baicalin, forsythin and emodin, and the peak areas of baicalin, forsythin and emodin The RSDs were 0.10%, 0.31%, and 0.13%, respectively, and the precision met the requirements of Veterinary Pharmacopoeia.

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Abstract

The invention discloses a tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation and a preparation process thereof. The traditional Chinese herbal medicine preparation is prepared from the following raw materials in parts by weight: 10-25 parts of scutellaria baicalensis, 10-25 parts of fructus forsythia, 10-25 parts of rheum officinale and 10-25 parts of dioscorea bulbifer. The preparation process comprises the following steps: weighing the raw material medicines according to formula weights, adding distilled water which is 20 times the total weight ofthe raw material medicines, and performing water bath for three times at 100 DEG C, and concentrating, thereby obtaining the traditional Chinese herbal medicine preparation, wherein the water bath lasts for one hour each time, and the alcohol precipitation concentration is 60%. The traditional Chinese herbal medicine preparation has the functions of clearing heat and removing toxins, preventing bacteria and preventing stress as well as regulating body immunity, the disease resistance of tilapia mossambica can be effectively improved, moreover the traditional Chinese herbal medicine preparationis low in medicine residue, medicine residue standard exceeding can be avoided, and drug resistance of pathogenic bacteria can be avoided; and besides, by adopting the preparation process disclosed by the invention, the contents of effective components of the tilapia mossambica streptococcicosis resistant traditional Chinese herbal medicine preparation can be effectively increased, so that the medicinal effect can be improved, the preparation process is simple, and the cost can be lowered.

Description

technical field [0001] The invention belongs to the technical field of preventing and treating tilapia streptococcosis, in particular to an anti-tilapia streptococcosis Chinese herbal medicine preparation and a preparation process thereof. Background technique [0002] Tilapia streptococcosis is a bacterial infectious disease caused by Streptococcus iniae and Streptococcus agalactiae as the main pathogens. Generally outbreaks occur in summer and autumn. Unfavorable environmental factors such as temperature, oxygen content, and ammonia content, as well as stress conditions such as poor feeding conditions and over-density breeding, all create favorable conditions for the prevalence of Streptococcus infection. The traditional treatment method is to add antibiotics to the feed. Long-term use of such drugs will not only pollute the ecological environment, but also lead to drug-induced diseases and drug residues, making pathogenic bacteria resistant to drugs, and the control effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61P31/04
CPCA61K36/539A61K36/634A61K36/708A61K36/8945A61K2236/331A61K2236/39A61K2236/55A61P31/04A61K2300/00
Inventor 胡庭俊谷俐媛梁万文宋漫玲施君
Owner GUANGXI UNIV
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