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A medium for large-scale high-density cultivation of green algae and its cultivation method

A technology of expansion culture medium and culture medium, which is applied in the field of plant cultivation, and can solve problems such as easy pollution, culture failure, and low culture density

Active Publication Date: 2021-07-30
海南源泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some bottlenecks in the cultivation of microalgae: 1. The culture density is low, which directly affects the success rate of continued propagation and the effect of use; And, it requires operating experience and is very troublesome; 3. It is very easy to pollute during the cultivation process, resulting in failure of cultivation, and it is impossible to achieve continuous expansion for a long time; 4. Algae cultivation depends on conditions such as light and water temperature, which requires professional technology, and the algae generator is expensive

Method used

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  • A medium for large-scale high-density cultivation of green algae and its cultivation method
  • A medium for large-scale high-density cultivation of green algae and its cultivation method
  • A medium for large-scale high-density cultivation of green algae and its cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Cultivating 100L of Chlorella vulgaris in indoor seawater includes the following steps:

[0035] (1) Disinfect and clean the cultivation generator.

[0036] (2) Seawater preparation: Seawater was filtered with 500-mesh gauze, disinfected with sodium hypochlorite, and the supernatant liquid was pumped out after 2 days for use.

[0037] (3) Culture medium configuration method:

[0038] Inorganic salt organic stock solution, 1000ml distilled water contains the following components:

[0039]

[0040] Trace element stock solution, 100ml distilled water contains the following ingredients:

[0041]

[0042] Growth factor element stock solution, 100ml distilled water contains the following ingredients:

[0043]

[0044] Booster buffer stock solution, 200ml distilled water contains the following ingredients:

[0045] Dipotassium hydrogen phosphate 8g

[0046] Potassium dihydrogen phosphate 2g

[0047] The expansion culture medium includes the following components ...

Embodiment 2

[0056] Cultivating 50L Chlorella pyrenoidosa in outdoor river water includes the following steps:

[0057] (1) Disinfect and clean the cultivation generator.

[0058] (2) River water preparation: the river water was filtered with 500 mesh gauze, disinfected with sodium hypochlorite, and the supernatant liquid was drawn after 2 days for use.

[0059] (3) Culture medium configuration method:

[0060] Inorganic salt organic stock solution, 1000ml distilled water contains the following components:

[0061]

[0062] Trace element stock solution, 100ml distilled water contains the following ingredients:

[0063]

[0064] Growth factor element stock solution, 100ml distilled water contains the following ingredients:

[0065]

[0066]

[0067] Booster buffer stock solution, 200ml distilled water contains the following ingredients:

[0068] Dipotassium hydrogen phosphate 8g

[0069] Potassium dihydrogen phosphate 2g

[0070] The expansion culture medium includes the f...

Embodiment 3

[0078] Cultivate 100L Scenedesmus obliques in outdoor tap water, comprising the following steps:

[0079] (1) Disinfect and clean the cultivation generator.

[0080] (2) Tap water preparation: Tap water should be aerated for more than 6 hours before use.

[0081] (3) Culture medium configuration method:

[0082] Inorganic salt organic stock solution, 1000ml distilled water contains the following components:

[0083]

[0084] Trace element stock solution, 100ml distilled water contains the following ingredients:

[0085]

[0086] Growth factor element stock solution, 100ml distilled water contains the following ingredients:

[0087]

[0088] Booster buffer stock solution, 200ml distilled water contains the following ingredients:

[0089] Dipotassium hydrogen phosphate 10g

[0090] Potassium dihydrogen phosphate 3g

[0091] The expansion culture medium includes the following components in volume percentage: 25% inorganic salt organic substance stock solution, 0.15...

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PUM

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Abstract

The invention discloses a culture medium for large-scale high-density cultivation of green algae, which includes a basic medium and an expansion medium, and the basic medium includes an inorganic salt organic substance stock solution, a trace element stock solution, a growth factor element stock solution and a synergistic buffer agent stock solution; the expansion medium is obtained by adding the base medium to the expansion water and stirring evenly. The culture medium of the invention contains the active ingredients required in the cultivation process of green algae, and the expansion speed is fast, the growth of green algae is stable, and it is not easy to age and die. The invention can realize high-density and low-cost expanded cultivation of green algae, and the expanded green algae has activity and can be bred in large quantities.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a technique for expanding cultivation of green algae, in particular to a medium for large-scale and high-density cultivation of green algae and a cultivation method thereof. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. Chlorella, Scenedesmus, Oocyst, and Chlorella are currently common types of cultured microalgae in green algae. They are rich in protein, vitamins, minerals, and some biologically active substances. They are used in health food, biological bait, and sewage Treatment, feed addition, bio-energy and other aspects have extensive development value. Howeve...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12M1/36C12R1/89
CPCC12M21/02C12M23/02C12M23/38C12M41/24C12N1/12
Inventor 王绥标张姝原居林李义书
Owner 海南源泉生物科技有限公司
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