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Preparation method and device for cephalosporin acylase immobilized enzyme

A technology for cephalosporin acylase and enzyme immobilization, which is applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of enzyme inactivation, poor mechanical properties, etc. Loss of enzymatic activity, effect of improving mechanical strength and resilience

Active Publication Date: 2018-09-04
陕西省生物农业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still disadvantages such as poor mechanical properties and easy inactivation of enzymes during the immobilization process. The key to the success of immobilizing enzymes is to choose a good carrier.

Method used

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  • Preparation method and device for cephalosporin acylase immobilized enzyme
  • Preparation method and device for cephalosporin acylase immobilized enzyme

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Effect test

Embodiment 1

[0027] A preparation method for cephalosporin acylase immobilized enzyme, comprising the following steps:

[0028](1) Preparation of a three-dimensional elastic carrier: the composite oil phase solution and the composite water phase solution are independently loaded into a multifunctional temperature-controlled water bath device with a volume ratio of 1:1, wherein the composite oil phase solution includes according to weight percentage: 7% carbon tetrachloride, 14% isopropanol, 15% silicone oil, 9% ether, 1% soybean isoflavones, 15% turpentine, 1% fluorocarbon surfactant, and the balance is wood wax oil. As an antioxidant, soybean isoflavones can prevent the autoxidation of oil phase components, and fluorocarbon surfactants can significantly improve the dispersibility of the composite oil phase solution in the composite water phase solution, thereby improving the uniformity of the prepared three-dimensional elastic carrier. The composite aqueous phase solution includes: 8% pol...

Embodiment 2

[0035] A preparation method for cephalosporin acylase immobilized enzyme, comprising the following steps:

[0036] (1) Preparation of a three-dimensional elastic carrier: the composite oil phase solution and the composite water phase solution are independently loaded into a multifunctional temperature-controlled water bath device with a volume ratio of 1:1, wherein the composite oil phase solution includes according to weight percentage: 8.5% carbon tetrachloride, 15% isopropanol, 16% silicone oil, 10% ether, 1.5% soybean isoflavones, 16% turpentine, 2% fluorocarbon surfactant, and the balance is wood wax oil. As an antioxidant, soybean isoflavones can prevent the autoxidation of oil phase components, and fluorocarbon surfactants can significantly improve the dispersibility of the composite oil phase solution in the composite water phase solution, thereby improving the uniformity of the prepared three-dimensional elastic carrier. The composite aqueous phase solution includes: ...

Embodiment 3

[0043] A preparation method for cephalosporin acylase immobilized enzyme, comprising the following steps:

[0044] (1) Preparation of a three-dimensional elastic carrier: the composite oil phase solution and the composite water phase solution are independently loaded into a multifunctional temperature-controlled water bath device with a volume ratio of 1:1, wherein the composite oil phase solution includes according to weight percentage: 10% carbon tetrachloride, 16% isopropanol, 17% silicone oil, 11% ether, 2% soybean isoflavones, 17% turpentine, 3% fluorocarbon surfactant, and the balance is wood wax oil. As an antioxidant, soybean isoflavones can prevent the autoxidation of oil phase components, and fluorocarbon surfactants can significantly improve the dispersibility of the composite oil phase solution in the composite water phase solution, thereby improving the uniformity of the prepared three-dimensional elastic carrier. The composite aqueous phase solution includes: 10%...

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Abstract

The invention discloses a preparation method and device for a cephalosporin acylase immobilized enzyme, and belongs to the technical field of biology. The preparation method comprises the steps that (1) composite oil phase solution and composite aqueous phase solution are emulsified to prepare a three-dimensional elastic carrier; (2) the three-dimensional elastic carrier is activated by utilizing3-dimethylaminopropylamine so as to increase adhesive force to the enzyme; (3) cephalosporin acylase and the three-dimensional elastic carrier are subjected to crosslinking modification by utilizing didextranacetaldehyde; and (4) a crosslinking modification enzyme is subjected to gradient temperature increasing aftertreatment. In a word, the cephalosporin acylase immobilized enzyme prepared through the preparation method and device has the advantages of being high in enzyme binding force, good in stability, high in mechanical property, high in enzyme activity, long in half-life period and thelike.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and a device for immobilizing cephalosporin acylase. Background technique [0002] Cephalosporin acylase is a class of antibiotics that can catalyze cephalosporin C (CPC) and glutaryl-7-aminocephalosporanic acid (GL-7ACA) to generate 7-aminocephalosporanic acid (7-ACA) for industrial use enzyme. However, as a free enzyme, it has disadvantages such as environmental sensitivity, unstable properties, easy inactivation in enzymatic reactions, and difficult separation and purification of products. Therefore, immobilized enzyme technology is often used in the prior art to solve this defect. [0003] Immobilized enzyme technology was produced in the 1960s and has been widely used in industrial production in the field of biocatalysis. Enzyme immobilization methods can be divided into four categories: adsorption, embedding, covalent binding, and cross-linki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C12N11/02C12N9/80C12M1/40C12M1/38C12M1/02
CPCC12M27/02C12M41/18C12N9/80C12N11/02C12N11/08C12N11/10C12Y305/01093
Inventor 秦涛陈卫锋徐升运赵文娟刘成更
Owner 陕西省生物农业研究所
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