High performance liquid chromatography for determinating taurine content based on differential detector

Abstract

The invention belongs to the technical field of medicine analysis, and particularly relates to a high performance liquid chromatography for determinating the taurine content of taurine granules or taurine eye drops based on a differential detector. According to the high performance liquid chromatography for determinating the taurine content based on the differential detector, high performance liquid chromatography is combined with a differential refraction detector to establish a high performance liquid chromatography-differential detection method for directly determining the taurine content;with an NH2 column as a separation column, detection is performed by using a differential refraction display; in the whole detection process, use of a carrier gas is not required and derivatization oftaurine is not required; in addition, no intermediate reaction process exists, the linear range is wide, and the taurine content can be directly determined easily, conveniently, quickly and accurately; by the high performance liquid chromatography, determination of the taurine content of the taurine granules and the taurine eye drops can be achieved.

Description

Technical field [0001] The invention belongs to the technical field of pharmaceutical analysis, and specifically relates to a high performance liquid chromatography method for measuring the taurine content in taurine particles or taurine eye drops based on a refractive index detector. Background technique [0002] Taurine, also known as β-aminoethanesulfonic acid, with the molecular formula C 2 H 7 NO 3 S, with a molecular weight of 125.15, is a sulfur-containing non-protein amino acid that was first isolated from bezoar. Taurine has a small molecule, does not have a phenyl group, has stable chemical properties, and is insoluble in organic solvents such as ether. It exists in a free state in the body and does not participate in the biosynthesis of proteins in the body. However, it interacts with cystine and cysteine. The metabolism of amino acid is closely related and is an important nutrient for humans and animals. It exists in almost all organs of mammals in free form, and it ...

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Problems solved by technology

Potentiometric titration is usually suitable for the determination of API samples that do not contain interfering substances or have few impurities. The spectrophotometric method after the color reaction of acetylacetone also requires a color reaction, and the operation is cumbersome. It is difficult to have an intuitive view of the reaction process and reaction conversion rate. The judgment of taurine will affect the accuracy of taurine determination; the principle of high performance liquid chromatography-evaporative light scattering detection method is to atomize the column eluent to form an aerosol, and then evaporate the solvent in the heated drift tube, and finally the remaining The non-volatile solute particles are detected in the light scattering detection cell, which requires high-pressure nitrogen or air as the carrier gas, which is inconvenient to use, the stability of the carrier gas, the atomization process, and the thermal stability of the sample to be tested will all affect the accuracy of the measurement; mass spectrometry The method has strong specificity and high accuracy, but it is expensive and the operation of the instrument is complicated. It is often used for the qualitative or quantitative determination of substance purity, structure or unknown samples, but it is used for routine testing of conventional known samples, and the cost of instrument use and test time Relatively high, which is not conducive to the saving of inspection costs and the improvement of inspection efficiency in conventional laboratories

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