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A method for screening the role of microRNAs in the adaptation of brown planthopper to rice resistance

A brown planthopper, survival rate technology, applied in the field of bioengineering

Active Publication Date: 2019-09-03
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the relationship between MicroRNAs and resistance adaptation in N. lugens.

Method used

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  • A method for screening the role of microRNAs in the adaptation of brown planthopper to rice resistance
  • A method for screening the role of microRNAs in the adaptation of brown planthopper to rice resistance
  • A method for screening the role of microRNAs in the adaptation of brown planthopper to rice resistance

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Embodiment 2

[0069] 1) Synthesis of miRNA mimic, miRNA inhibitor, and control strands Through differential analysis of miRNA expression, we found 26 miRNAs with large differential expression. Among them, the miRNA expression level of the BPH population (biotype I population) on the susceptible rice TN1 was higher, and the miRNA expression level of the NPH population (biotype Y population) on the insect-resistant rice YHY15 was lower. The differentially expressed miRNAmimic, inhibitor, negative control for mimic (NCm) and negative control for inhibitor (NCi) synthesized in this experiment were designed and synthesized by Guangzhou Saizhe Biotechnology Co., Ltd.

[0070] 2) Microinject and artificially feed miRNA mimic and miRNA inhibitor to BPH to verify the function of miRNAs.

[0071] The purified miRNA mimic and miRNA inhibitor were slowly injected into the intersegmental membrane of the prothorax and mesothorax ventral surface of the newly eclosioned brown planthopper using the instrume...

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Abstract

The invention discloses a method for screening the adaptability of MicroRNAs in brown planthopper's resistance to rice, and relates to bioengineering technology. The method comprises the following steps: 1. collection of samples; 2. sequencing of small RNAs; 3. preprocessing of sequencing raw data; 4. sequence alignment; Synthesis of miRNA inhibitor and control strand; ⑨microinjection and artificial feeding of miRNA mimic and miRNA inhibitor to brown planthopper to verify the function of miRNAs; ⑩preliminary study on the adaptation of brown planthopper to rice resistance after microinjection and artificial feeding. The present invention uses high-throughput sequencing technology to deeply sequence the small RNAs of two brown planthopper populations, and through subsequent bioinformatics analysis to identify and predict miRNAs, compare the two brown planthoppers on insect-sensitive rice TN1 and insect-resistant rice YHY15 Differences between populations, analysis, screening and discovery of miRNAs related to host resistance adaptation, provide new methods for rice insect resistance breeding.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to a method for screening the adaptability of MicroRNAs in brown planthopper's resistance to rice. Background technique [0002] Rice (Oryza sativa) is one of the world's major food crops, and more than half of the world's population uses rice as a staple food. Experts estimate that by 2025, the world's rice production will have to increase by 60% to meet the demand. my country is a big country in rice production. The annual rice planting area is about 30 million hectares, accounting for 1 / 4 of the world's total rice planting area. Therefore, the safety of rice planting is inseparable from my country's economic development and people's lives. [0003] Brown planthopper (Nilaparvata lugens BPH) is an insect belonging to the family Homoptera (Homoptera: Delphacidae), also known as brown rice lice. The brown planthopper mainly absorbs the sap of the rice phloem, and the first to be dam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6869G16B30/00G16B20/00
Inventor 闸雯俊游艾青徐华山李培德李三和周雷刘凯陈志军杨国才刘林
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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