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A kind of mugwort leaf extract and its preparation method and application

Artemisia argyi extract and Artemisia argyi, applied in the field of artemisia argyi extract and its preparation

Active Publication Date: 2020-06-09
湖北艾艾贴健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the killing of agricultural pests (such as aphids and armyworms) by Artemisia argyi extracts or active compounds

Method used

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  • A kind of mugwort leaf extract and its preparation method and application
  • A kind of mugwort leaf extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Get dried Artemisia argyi (1.0kg) and pulverize, after sterilizing, place in aseptic fermenter, then add glucose (100g) and yeast powder (30g), after mixing evenly, fermenter is sealed, in At 20-25°C, ferment naturally for 20 days to obtain a fermented product;

[0032] (2) Soak the fermented product obtained in step (1) with acetone (8L) for 24 hours, filter, and after the filtrate is concentrated, absorb it through AB-8 type macroporous resin, elute with a volume fraction of 60% ethanol solution, collect 6 times the column volume eluate, then decolorized with activated carbon, concentrated, and dried to obtain the Artemisia argyi extract (hereinafter referred to as product A).

Embodiment 2

[0034] (1) Get dry Folium Artemisiae Argyi (1.0kg) and pulverize, after sterilizing, place in aseptic fermenter, then add glucose (150g) and yeast powder (50g), after mixing evenly, fermenter is sealed, in At 20-25°C, ferment naturally for 25 days to obtain a fermented product;

[0035] (2) Soak the fermented product obtained in step (1) with acetone (10L) for 20 hours, filter, and after the filtrate is concentrated, it is absorbed by D101 type macroporous resin, eluted with ethanol solution with a volume fraction of 70%, and collected 5 times column volume eluate, decolorized by activated carbon, concentrated, and dried to obtain the Artemisia argyi leaf extract (hereinafter referred to as product B).

Embodiment 3

[0037] (1) Get dry Folium Artemisiae Argyi (1.0kg) and pulverize, soak with acetone (8L) for 24 hours, filter, after the filtrate is concentrated, absorb through AB-8 type macroporous resin, be 60% ethanol solution elution with volume fraction, The eluate was collected for 6 times of column volume, decolorized by activated carbon, concentrated, and dried to obtain product C (no compound of formula I was found in product C by TLC).

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Abstract

The invention relates to a mugwort extract and preparation and application methods thereof. The preparation method of the mugwort extract comprises the following steps of (1) crushing and sterilizingdried mugwort leaves, then placing the crushed and sterilized mugwort leaves into a sterile fermentation cylinder, then uniformly mixing in glucose and yeast powder, and sealing up the fermentation cylinder for natural fermentation for 20-25 days to obtain fermented materials; (2) soaking the fermented materials of the step (1) in acetone, then filtering the mixture, concentrating and absorbing filter liquor through macroporous resin, eluting the filter liquor through ethanol solution of with 60-70% of alcohol by volume, collecting eluent of 5-6 times of column, and then performing activated carbon decoloration, concentration and drying to obtain the mugwort extract.

Description

technical field [0001] The invention belongs to the field of extraction, and in particular relates to an extract of mugwort leaves, a preparation method and application thereof. Background technique [0002] Artemisia argyi is the dry leaf of the perennial herb Artemisia argyi Levl. et Vant. in the family Compositae. At present, more than 100 compounds have been isolated from Artemisia argyi, including terpenoids, flavonoids, phenylpropanoids, and aromatic acids. (aldehydes), steroids and fatty acids and other chemical components. It shows a wide range of biological activities, such as analgesia, anti-inflammation, anti-coagulation, anti-tumor, etc. Among them, the volatile oil of Artemisia argyi leaves also has a certain effect of removing mites. However, there is no report on the killing of agricultural pests (such as aphids and armyworms) by extracts or active compounds of leaves of Artemisia argyi. The invention provides the application of fermented mugwort leaf extrac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18A01N43/90A01P5/00A01P7/04C12R1/645
CPCA01N43/90C12P17/188C12P19/626
Inventor 周培束影蒋泽慧
Owner 湖北艾艾贴健康科技有限公司
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