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Induction culture medium for tomato tissue culture, tissue culture method and application

A technology for inducing medium and tissue culture, applied in the directions of application, horticultural methods, botanical equipment and methods, etc., can solve the problems of water resources and fertilizer waste, quality decline, and high incidence of vegetable disease

Inactive Publication Date: 2018-09-21
孟静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the production of greenhouse vegetables mainly relies on a large amount of fertilizer and water input to increase production, it not only causes waste of water resources and fertilizers, but also leads to a high incidence of vegetables and a decline in quality.
At the same time, it is common to apply less or no organic fertilizer, partial application of nitrogen fertilizer, heavy application of phosphorus fertilizer, and less or no application of potassium fertilizer. This long-term nutritional status of high nitrogen, rich phosphorus, and low potassium leads to imbalanced nutrient supply. It has become one of the main factors limiting the improvement of tomato quality in the current greenhouse
[0004] At present, most of the methods for increasing the content of lycopene in tomato focus on the reasonable supply of nitrogen, phosphorus, potassium and water, but there is no induction of tomato tissue culture seedlings from the direction of tissue culture, and then improve the production of tomato in tomato tissue culture seedlings. Method of red pigment content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Cultivation of tomato tissue culture seedlings

[0046] The tomato tissue culture process is carried out in a culture room. Set the culture temperature of the tissue culture room at 25 °C, the humidity at 50%, the light intensity at 2000 Lx, the light period of 16 hours, and the dark period of 8 hours.

[0047] Prepare the medium according to the following recipe:

[0048] Induction medium: MS basic medium + 3-indoleacetic acid 0.04mg / L+6-benzylaminoadenine 1.0mg / L+ methyl jasmonate 1.5mg / L+ polyvinylpyrrolidone 5mg / L+ agar 5g / L+ sucrose 30g / L; pH value is 6.0;

[0049] Rooting medium: MS basic medium + indole butyric acid 0.8 mg / L + agar 5 g / L + sucrose 30 g / L.

[0050] The leaves of Jingdan No. 1 tomato were used as explants, rinsed thoroughly with tap water, then soaked in 5% Tween-20 for 5 minutes, rinsed 3-4 times with sterilized distilled water, 0.1% mercuric chloride (HgCl 2 ) Surface sterilize for 4 minutes, rinse 4-5 times with sterile distilled water. Th...

Embodiment 2

[0052] Cultivation of tomato tissue culture seedlings

[0053] The tomato tissue culture process is carried out in a culture room. Set the tissue culture room culture temperature to 24 °C, humidity to 55%, light intensity to 2500 Lx, 15-hour photoperiod, and 9-hour dark period.

[0054] Prepare the medium according to the following recipe:

[0055] Induction medium: MS basic medium+ 3-indoleacetic acid 0.05mg / L+6-benzylaminoadenine 1.5mg / L+ methyl jasmonate 2.5mg / L+ polyvinylpyrrolidone 8mg / L+ agar 7g / L+ sucrose 35g / L, the pH value is 5.5;

[0056] Rooting medium: MS basic medium + indole butyric acid 1.0 mg / L + agar 8 g / L + sucrose 35 g / L.

[0057] The leaves of Jingdan No. 1 tomato were used as explants, rinsed thoroughly with tap water, then soaked in 5% Tween-20 for 6 minutes, rinsed 3-4 times with sterilized distilled water, 0.1% mercuric chloride (HgCl) 2 ) Surface sterilize for 4 minutes, rinse 4-5 times with sterile distilled water. The leaf tissue was cut into a...

Embodiment 3

[0059] Cultivation of tomato tissue culture seedlings

[0060] The tomato tissue culture process is carried out in a culture room. Set the tissue culture room culture temperature to 26 °C, humidity to 60%, light intensity to 3000 Lx, 14-hour photoperiod, and 10-hour dark period.

[0061] Prepare the medium according to the following recipe:

[0062] Induction medium: MS basic medium+ 3-indoleacetic acid 0.02mg / L+6-benzylaminoadenine 0.8mg / L+ methyl jasmonate 3.7mg / L+ polyvinylpyrrolidone 10 mg / L+ agar 8g / L+ sucrose 40g / L, pH value is 6.5;

[0063] Rooting medium: MS basic medium + indole butyric acid 1.2 mg / L + agar 10 g / L + sucrose 40 g / L.

[0064] The leaves of Jingdan No. 1 tomato were used as explants, rinsed thoroughly with tap water, then soaked in 5% Tween-20 for 8 minutes, rinsed 3-4 times with sterilized distilled water, 0.1% mercuric chloride (HgCl) 2 ) Surface sterilize for 4 minutes, rinse 4-5 times with sterile distilled water. The leaf tissue was cut into a...

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PUM

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Abstract

The invention provides an induction culture medium for tomato tissue culture, a tomato tissue culture seedling cultured by using the induction culture medium, and a culture application of the tomato tissue culture seedling. The induction culture medium takes an MS basal culture medium as a foundation and comprises 0.02-0.05mg / L 3-indoleacetic acid, 0.5-1.5mg / L 6-benzylaminoadenine, 1.5-3.7mg / L methyl jasmonate, 5-10mg / L polyvinylpyrrolidone, 5-8g / L agar and 30-40g / L sucrose. Methyl jasmonate and polyvinylpyrrolidone are added into the induction culture medium; the expression of a lycopene genein a tomato tissue culture tissue is completed excited; after culture of the obtained tomato tissue culture seedling, produced tomatoes have a very high lycopene content; and the quality of the tomatoes is improved.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an induction medium for tomato tissue culture and a tissue culture method and application. Background technique [0002] Lycopene is a natural pigment contained in plants. Mainly found in the ripe fruit of the nightshade tomato. It is currently one of the strongest antioxidants found in plants in nature. Lycopene is far more effective in scavenging free radicals than other carotenoids and vitamin E, and its rate constant for quenching singlet oxygen is 100 times that of vitamin E. It can effectively prevent various diseases caused by aging and weakened immunity, so it has attracted much attention. [0003] Lycopene is an important indicator to measure the quality of tomatoes. At present, tomato greenhouse cultivation is an important measure to increase tomato yield. However, the production of greenhouse vegetables mainly relies on a large amount of fertilizer and wate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 孟静
Owner 孟静
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