Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for rapid flocculation of co-cultivated Monospora under neutral conditions

A neutral condition, single needle algae technology, applied in microorganism-based methods, single-cell algae, biochemical equipment and methods, etc., can solve the problems of unfeasible industrial promotion, high operating costs, secondary pollution, etc.

Active Publication Date: 2021-09-14
KUNMING UNIV OF SCI & TECH
View PDF17 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on chemical flocculation harvesting of microalgae started earlier, but this kind of technology is not suitable for large-scale application. The main reasons are as follows: First, the residues of metal ions and polymers will cause secondary pollution. problems; secondly, the requirements for pH and other conditions are high, and additional treatment is required; finally, the operation cost is high, and it does not have the feasibility of industrial promotion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for rapid flocculation of co-cultivated Monostipella under neutral conditions, comprising the following steps:

[0029] (1) Under the conditions of 25±1°C, light intensity of 2500-4000lux, and shaker speed of 150r / min, two strains of Monospina were autotrophically cultured on BG-11 basic medium. Monoraphidium sp. (FACHB-1821) and Monoraphidium sp. (FACHB-1853), collect algae cells after the growth of Monopodella to the late logarithmic phase, and use it as co-cultivation seed solution;

[0030] (2) Insert the seed solution of two strains of Monospina in fresh BG-11 medium at the same time, dilute and resuspend the algal cells, and co-culture under light conditions. The light intensity is 2500-4000lux, the rotation speed of the shaker is 150r / min, and the culture temperature is 25±1°C. The total cell density of the two strains of microalgae in the co-culture system was 4.00×10 6 cells / mL, Monopodella Monoraphidium sp. (FACHB-1821) and Monoraphidium The init...

Embodiment 2

[0033] A method for rapid flocculation of co-cultivated Monostipella under neutral conditions, comprising the following steps:

[0034](1) Under the conditions of 25±1°C, light intensity of 2500-4000lux, and shaker speed of 150r / min, two strains of Monospina were autotrophically cultured on BG-11 basic medium. Monoraphidium sp. (FACHB-1821) and Monoraphidium sp. (FACHB-1853), collect algae cells after the growth of Monopodella to the late logarithmic phase, and use it as co-cultivation seed solution;

[0035] (2) Insert the seed solution of two strains of Monospina in fresh BG-11 medium at the same time, dilute and resuspend the algal cells, and co-culture under light conditions. The light intensity is 2500-4000lux, the rotation speed of the shaker is 150r / min, and the culture temperature is 25±1°C. The total cell density of the two strains of microalgae in the co-culture system was 4.00×10 6 cells / mL, Monopodella Monoraphidium sp. (FACHB-1821) and Monoraphidium The initi...

Embodiment 3

[0038] A method for rapid flocculation of co-cultivated Monostipella under neutral conditions, comprising the following steps:

[0039] (1) Under the conditions of 25±1°C, light intensity 2500-4000lux, and shaker speed 150r / min, two strains of Monospina were autotrophically cultured on BG-11 basal medium. Monoraphidium sp. (FACHB-1821) and Monoraphidium sp. (FACHB-1853), collect algae cells after the growth of Monopodella to the late logarithmic phase, and use it as co-cultivation seed solution;

[0040] (2) Insert the seed solution of two strains of Monospina in fresh BG-11 medium at the same time, dilute and resuspend the algal cells, and co-culture under light conditions. The light intensity is 2500-4000lux, the rotation speed of the shaker is 150r / min, and the culture temperature is 25±1°C. The total cell density of the two strains of microalgae in the co-culture system was 4.00×10 6 cells / mL, Monopodella Monoraphidium sp. (FACHB-1821) and Monoraphidium The initial ce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for rapid flocculation of co-culturing Monospina under neutral conditions. Firstly, two different strains of Monospina are cultured respectively, and the algal cells are collected after growing to the late stage of logarithmic growth phase for co-cultivation. After the stable period, the pH value of the algae solution was adjusted to 7.0, and the algae cells aggregated rapidly. The present invention quickly flocculates microalgae under neutral conditions, can improve the flocculation performance of algae cells, reduce the use of chemical flocculants, avoid polluting algae cells, improve the efficiency of algae cell harvesting, and reduce the cost of algae cell harvesting, thereby greatly Reduce the cost of biodiesel production and development and application.

Description

technical field [0001] The invention belongs to the technical field of energy microalgae, and in particular relates to a method for rapid flocculation of co-culturing Monopodella under neutral conditions. Background technique [0002] As an important raw material of biodiesel oil, microalgae has attracted extensive attention from all over the world. At present, large-scale cultivation of microalgae to produce oil is technically feasible, but due to the constraints of high production costs, large-scale industrial production is not yet possible. The high cost of harvesting microalgae biomass has always been one of the main bottlenecks restricting the development of microalgae oil biodiesel. In particular, the harvesting of algal biomass is more challenging due to the small size of microalgal cells, the small difference in density between algal cells and the culture solution, and the low concentration of biomass. It is estimated that the cost of microalgae harvesting accounts...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 赵鹏赵飞燕肖钧木
Owner KUNMING UNIV OF SCI & TECH