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Deep-sea bacillus and application thereof to inducing adhesion of juvenile mollusk of mytilus crassitesta

A deep-sea bacillus and bacillus technology, applied in the application, bacteria, climate change adaptation and other directions, can solve the problem that the natural seed cannot meet the development of the thick-shelled mussel aquaculture industry.

Active Publication Date: 2018-10-02
SHANGHAI OCEAN UNIV
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Problems solved by technology

In recent years, due to the over-exploitation of fishery resources, natural seedlings cannot meet the needs of the development of the mussel culture industry.

Method used

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  • Deep-sea bacillus and application thereof to inducing adhesion of juvenile mollusk of mytilus crassitesta

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Embodiment Construction

[0008] The specific implementation manner of the present invention will be further described in detail through the description of the best embodiment below.

[0009] Isolation and storage of strains:

[0010] The deep-sea bacillus (Bacillus sp.29R7-12) involved in the present invention is derived from oceanic sediments (E 142.20°, N 41.18°) near the Shimokita Peninsula at an altitude of -1180 meters. Pure colonies were isolated and purified using ZoBell 2216E solid medium, and were uploaded to the NCBI database after comparison with the nucleic acid data in GENEBANK (http: / / www.ncbi.nlm.nih.gov / ) through 16S rRNA gene sequencing. The final name of the bacterium was Bacillus, and the obtained serial number was MG252257. The bacterium was preserved in the China Center for Type Culture Collection (CCTCC) with the preservation date of January 29, 2018, and the preservation number was CCTCC M 2018065.

[0011] The present invention relates to the induction experiment of deep-sea b...

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Abstract

The invention discloses deep-sea bacillus which has the preservation number of CCTCC M 2018065, and is classified and named as Bacillus sp. A microbial envelope formed by bacteria has a relatively high induction activity on the adhesion of a juvenile mollusk of mytilus crassitesta. The invention further relates to application of the deep-sea bacillus to inducing the adhesion of the juvenile mollusk of the mytilus crassitesta.

Description

technical field [0001] The present invention relates to the application of a strain of deep-sea bacteria in inducing the attachment of thick-shelled mussel juveniles, in particular to the isolation, purification, identification and preservation of deep-sea bacteria Bacillus sp. The effect of microbial film formed under the environment on the attachment of spat of Mussel mussels. Background technique [0002] Thick-shelled mussel (Mytilus coruscus), belonging to the mollusca (Mollusca), bivalves (Bivalvia), mytilodia (Mytilodia), mytilidae (Mytilidae), is an important marine shellfish culture species in my country, distribution Along the coasts of the Yellow Sea, the Bohai Sea and the East China Sea. Thick-shelled mussels have high nutritional value and have broad breeding prospects and market development potential. In the life history of thick-shelled mussels, in the later stage of planktonic life, eye-spot larvae will attach and metamorphose into juveniles, and eventually ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01K61/54C12R1/07
CPCA01K61/54C12N1/205C12R2001/07Y02A40/81
Inventor 杨金龙梁箫彭莉华陈珂魏玉利方家松
Owner SHANGHAI OCEAN UNIV
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