Quantitative cell enucleating method

A nuclear method and cell technology, which is applied in the field of micromanipulation at the cell level, can solve the problems of general enucleation volume range, poor consistency of development results, and low cloning operation efficiency, and achieve the goal of improving consistency and cloning operation success rate Effect

Pending Publication Date: 2018-10-02
NANKAI UNIV
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Blind aspiration enucleation is a commonly used enucleation method at present. It generally needs to absorb one quarter to one third of the amount of cytoplasm near the polar body to achieve enucleation. The range of enucleation volu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative cell enucleating method
  • Quantitative cell enucleating method
  • Quantitative cell enucleating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The oocytes used in this example were domestic pig oocytes taken from local slaughterhouses.

[0021] Oocytes are obtained as follows:

[0022] After the ovaries were removed from the slaughterhouse, they were transported to the laboratory within two hours in a thermos filled with normal saline at 35° to 37°. Immediately then wash twice at 37° with sterile saline containing 100 IU / L of penicillin and 50 mg / L of streptomycin. Oocytes were extracted from 2 to 6 mm diameter follicles on the ovary, and the extracted cells were washed three times with TL-Hepes-PVA and incubated for 42 hours in in vitro maturation (IVM) in a 39°, 5% carbon dioxide incubator . After IVM, cells were devitrified with 0.1% hyaluronidase. Finally, the cells were washed three times with M199 to obtain the oocytes used.

[0023] This example uses the NK-MR601 micromanipulator to operate, the device system includes two three-degree-of-freedom manipulator arms, one of which is used to load the ooc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a quantitative cell enucleating method, and belongs to the technical field of cell-level micromanipulation. The quantitative cell enucleating method is characterized in that during blind aspiration enucleation, the cytoplasm of an oocyte is found to tend to have elastic solid properties and the equilibrium pressure during the enucleation is linearly reduced, so that quantitative cell enucleation can be achieved through precise control of the equilibrium pressure during the enucleation process. By the quantitative cell enucleating method, the problem that the enucleationamount is difficult to control during the blind aspiration enucleation is solved.

Description

technical field [0001] The invention belongs to the technical field of micromanipulation at the cell level. Background technique [0002] The enucleation process of cells is a key step in biological operations such as nuclear transfer and cloning. The amount of enucleation directly affects the later developmental outcome of cells. Excessive amount of enucleation will accelerate the abnormal differentiation of recombinant embryos; too little amount of enucleation will cause polyploidy, abnormal cleavage and developmental retardation. Blind suction enucleation, as a common enucleation method at present, generally needs to suck one quarter to one third of the cytoplasm near the polar body to achieve enucleation. The amount of denucleation in the nucleation process is very random, resulting in poor consistency of later developmental results and low cloning efficiency. Therefore, it is necessary to design a quantitative enucleation method for cell enucleation, which is necessa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/075
CPCC12N5/0609
Inventor 赵新刘曜玮冯泽阳孙明竹崔茂盛
Owner NANKAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap