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A kind of honeysuckle mushroom extract with antiviral effect and preparation method and application thereof

A technology of honeysuckle vine and honeysuckle vine alcohol, which is applied in the field of natural medicinal chemistry and can solve the problems that there is no extract of honeysuckle vine to resist herpes virus

Active Publication Date: 2021-04-09
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no report that Lonicera vine extract has anti-herpes virus

Method used

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  • A kind of honeysuckle mushroom extract with antiviral effect and preparation method and application thereof
  • A kind of honeysuckle mushroom extract with antiviral effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 has the preparation method of the Lonicerae vine extract of antiviral effect

[0033] (1) get Lonicerae vine (collected from Linyi, Shandong), use 95% ethanol by volume fraction to carry out heating and reflux extraction for 2h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; The dosage ratio is 10mL: 1g;

[0034] (2) Put the alcoholic extract of Lonicerae vine into the D101 macroporous resin column, first use 3 times the volume fraction of the column volume as 5% ethanol aqueous solution to remove impurities; then use 5 times the volume fraction of the column volume as 35% ethanol aqueous solution; collect the eluted part with a volume fraction of 35% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the extract of Lonicerae vine.

Embodiment 2

[0035] Embodiment 2 has the preparation method of the Lonicerae vine extract of antiviral effect

[0036] (1) get Lonicerae vine (collected from Linyi, Shandong), heat and reflux extraction with 70% ethanol for 3h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; described ethanol and Lonicera vine alcohol The dosage ratio is 15mL: 1g;

[0037] (2) Enter the D101 macroporous resin column with the alcoholic extract of Lonicerae vine, first use 5 times of the column volume to elute the ethanol aqueous solution (i.e. pure water) with a volume fraction of 0% to remove impurities; then use 4 times of the column volume Elute with 30% ethanol aqueous solution; collect the eluted part with 30% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the extract of Lonicerae vine.

Embodiment 3

[0038] Embodiment 3 has the preparation method of the Lonicerae vine extract of antiviral effect

[0039] (1) get Lonicera vine (collected from Linyi, Shandong), heat and reflux extraction with 80% ethanol for 1h to obtain extract, concentrate the extract to obtain Lonicera alcohol extract; described ethanol and Lonicera vine alcohol The dosage ratio is 8mL: 1g;

[0040] (2) Put the alcoholic extract of Lonicera vine into the D101 macroporous resin column, first use 1 times the volume fraction of the column volume to elute and remove impurities with ethanol aqueous solution of 10%; then use 4 times the volume fraction of the column volume to be 40% ethanol aqueous solution; collect the eluting part with a volume fraction of 40% ethanol aqueous solution, concentrate and dry to obtain the effective part of macroporous resin column chromatography, that is, the extract of Lonicerae vine.

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Abstract

The invention relates to the technical field of natural medicine chemistry, and specifically discloses a honeysuckle vine extract with antiviral effect, a preparation method and application thereof. The preparation method of the Lonicera vine extract comprises the following steps: (1) taking the Lonicerae vine extract, extracting with alcohol, and concentrating the extract to obtain the Lonicera vine alcohol extract; (2) performing large-scale extraction of the Lonicera vine alcohol extract Porous resin column chromatography, obtains macroporous resin column chromatography effective part; Wherein, the concrete method of carrying out macroporous resin column chromatography in the step (2) is: upper macroporous resin column of honeysuckle vine alcohol extract, first use 1 to 5 times the column volume with a volume fraction of 0 to 10% ethanol aqueous solution to elute and remove impurities; then use 1 to 6 times the column volume to elute with an ethanol water solution with a volume fraction of 30 to 40%; collect a volume fraction of 30 to The fraction eluted with 40% ethanol aqueous solution was concentrated and dried to obtain the fraction effective for macroporous resin column chromatography. The honeysuckle vine extract prepared by the method has good anti-herpes virus effect.

Description

technical field [0001] The invention relates to the technical field of natural medicine chemistry, in particular to a honeysuckle vine extract with antiviral effect and its preparation method and application. Background technique [0002] Herpesviruses are a class of enveloped DNA viruses that mainly invade tissues of ectoderm origin, including skin, mucous membranes, and nerve tissues. The site of infection and the diseases it causes are various, and there is a tendency of latent infection, which seriously threatens human health. Herpes simplex virus is a representative species of herpes virus, which is divided into herpes simplex virus type I (HSV-I) and herpes simplex virus type II (HSV-II). Among them, herpes simplex virus type I (HSV-I) mainly causes skin, mucous membrane and organ infections other than the genitals; herpes simplex virus type II (HSV-II) mainly causes skin and mucous membrane infection in the genital area. Herpes simplex virus type II can cause genita...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61P31/22
CPCA61K36/07A61K2236/33A61K2236/55A61P31/22
Inventor 杨文强郭如男白丽君
Owner LINYI UNIVERSITY
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