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Preparation method and application of a hot pot food matrix papaverine standard substance

A standard substance, papaverine technology, applied in the preparation of hot pot food matrix papaverine standard substance, in the field of matrix standard substance, it can solve problems such as difficult to meet, expensive price, unsatisfactory monitoring, etc.

Active Publication Date: 2021-11-09
INTEGRATED TECH SERVICE CENT SANMING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the pure standard products of papaverine are imported, which are expensive and inconvenient to purchase, and are far from meeting the needs of domestic monitoring
Moreover, based on the inherent changeable characteristics of food and agricultural products, it is difficult to meet the growing requirements of various food and agricultural products by simply using traditional pure chemical standard detection methods.
At present, there is still a lack of papaverine biological reference materials for the quality control of illegally added non-edible substances in food. Through the development of reference materials for papaverine compounds in hot pot food, it is required that such papaverine biological reference materials have a certain degree of uniformity and Stability, expect to fill the vacancy in this aspect

Method used

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  • Preparation method and application of a hot pot food matrix papaverine standard substance
  • Preparation method and application of a hot pot food matrix papaverine standard substance
  • Preparation method and application of a hot pot food matrix papaverine standard substance

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Embodiment 1

[0021] 1 Materials and methods

[0022] 1.1 Instruments, utensils and reagents

[0023] 1.1.1 Instrument: API3500 liquid mass spectrometer, distribution electrospray ion source (ESI source), positive ion monitoring mode: Urel = 5%, k = 2; electronic balance: Class I, resolution 0.0001g, maximum allowable The error is ±0.05mg; the expanded uncertainty of the weighing balance is U=0.6mg (k=2).

[0024] 1.1.2 Vessels: 100mL volumetric flask: Grade A, with a maximum allowable error of ±0.10mL; 10mL volumetric flask (Grade A), with a maximum allowable error of ±0.015mL at 20°C; 1mL pipette (apparatus number JS2012002): Grade A , the maximum allowable error is ±1.0%; 10mL graduated pipette: Grade A, the maximum allowable error is ±0.05mL; 2mL graduated pipette: A grade, the maximum allowable error is ±0.012mL.

[0025] 1.1.3 Main reagents: papaverine standard substance: purity ≥ 98.00% ± 0.5%, Stanford Analytical Chemicals Inc, USA; chromatography grade reagents for acetonitrile a...

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Abstract

The invention belongs to the technical field of matrix standard substances for detection of illegally added non-edible substances in food, and in particular relates to a method for preparing hot pot food matrix papaverine standard substances. Including the following steps: (1) selecting negative hot pot food as a matrix sample; (2) adding papaverine standard substance to the matrix sample, stirring and mixing, and subpackaging to obtain matrix standard substance; (3) step (2) ) The obtained matrix standard substance was tested for homogeneity and stability. And the matrix standard substance that is made by described preparation method. And the purposes of described matrix standard substance. The uniformity and stability of the sample matrix standard substance prepared by the invention can meet the detection requirements, and the influence of matrix interference on the detection results is greatly reduced, providing quality control reference and ability evaluation basis for the detection of alkaloid compounds in poppy shells. The prepared reference material can be used for method validation and quality control of papaverine in hot pot food.

Description

technical field [0001] The invention belongs to the technical field of matrix standard substances for the detection of illegally added non-edible substances in food, in particular to a preparation method and application of a hot pot food matrix papaverine standard substance, and more specifically to a preparation method of a hot pot food matrix papaverine standard substance. And the matrix standard substance prepared by the method, and the application of the matrix standard substance. Background technique [0002] In recent years, cases of poppy shell ingredients found in food have occurred from time to time. Poppy husk is the dried and mature husk of Papaveraceae plants after harvesting opium. It contains more than 20 alkaloids, of which papaverine, morphine, codeine, thebaine, and narcotin are the main components. Adding opium poppy shells to food, its ingredients will cause great harm to people's physical and mental health, easily make consumers addicted, produce depende...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N30/06
CPCG01N1/28G01N30/06G01N2001/2893G01N2030/047
Inventor 张信仁张云程立军刘正才唐庆强
Owner INTEGRATED TECH SERVICE CENT SANMING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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