Method for identifying number of effective parents in grass carp intraspecific cross combinations
A technology of hybrid combination and parents, which can be used in fish farming, measuring devices, instruments, etc., and can solve the problem of the small number of effective parents
Inactive Publication Date: 2018-10-26
SHANGHAI OCEAN UNIV
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Problems solved by technology
[0005] The first object of the present invention is to provide a method for identifying the number of effective parents of grass carp intraspecies hybridization combinations in view of the problem that there are often few effective parents in the construction of grass carp intraspecies hybridization combinations
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[0018] The specific implementation modes provided by the present invention will be described in detail below in conjunction with the examples.
[0019] Taking Intraspecific Hybridization of Grass Carp in the Yangtze River, Pearl River and Heilongjiang Water System as an Example
[0020] 1) 90 groups of broodstock were selected from the Hanjiang population of the Yangtze River system, the Zhaoqing population of the Pearl River system and the Nenjiang population of the Heilongjiang water system preserved by the Grass Carp Genetics and Breeding Center of the Ministry of Agriculture, and each broodstock was injected with an electronic marker using the PIT electronic marker purchased from Guangdong Hongteng , obtain a mark number after scanning, so as to identify the source of parent fish when pairing and breeding, and cut the fin rays of each fish. In the winter of the first year, when the parents turned the ponds, 270 groups of 3 groups of three water systems were placed in 3 par...
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The invention relates to a method for identifying the number of effective parents in grass carp intraspecific cross combinations. The method includes the steps: 1) selecting parent fishes, marking theparent fishes and placing the parent fishes of different groups or strains needed for cross into the same parent pond in a first year to perform prenatal culture; 2) manually injecting hormone solution for acceleration of maturation in a second year, performing artificial fertilization to obtain fertilized ova, mixing the fertilized ova in the same combination and different families in a balancedmanner and placing the fertilized ova into the same circular hatching channel for hatching; 3) performing progeny test on the different combinations obtained in the step 2) by a paternity test method, determining the specific number of the effective parents in each combination offspring from cross parents and maintaining the combinations with more effective parents. The method has the advantagesthat molecular marker-assisted selection is combined with cross selection, maternal effects are decreased, cross parent contribution rate difference caused by different brood amount of the parents isdecreased, the number of the effective parents in each combination offspring is determined, and each combination can take cross advantages.
Description
technical field [0001] The invention relates to the technical field of aquatic germplasm resources, in particular to a method for identifying the number of effective parents of grass carp intraspecies hybrid combinations. Background technique [0002] Grass carp is a herbivorous fish with the highest aquaculture output in the world. The aquaculture output in my country accounts for more than 95% of the world's total output. Grass carp farming has a long history, which can be traced back to the Tang Dynasty in my country. Malaysia was the first to introduce grass carp from China and has been introduced to more than 40 countries. Due to the long sexual maturity time of grass carp, it is difficult to preserve species, so far no new species have been bred. Therefore, the germplasm problem has become the main bottleneck restricting the high-quality development of grass carp aquaculture. [0003] Hybridization is an important method for germplasm innovation, which can rapidly a...
Claims
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Login to View More IPC IPC(8): A01K61/10G01N33/00
CPCA01K61/10G01N33/00Y02A40/81
Inventor 沈玉帮李家乐徐晓雁张猛
Owner SHANGHAI OCEAN UNIV