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Cell-penetrating peptide as well as preparation method and application thereof

A technology of penetrating peptides and cells, applied in the field of biomedicine, can solve the problem of no unified conclusion, etc., and achieve the effects of low immunogenicity, high penetrating efficiency and remarkable penetrating effect.

Active Publication Date: 2018-10-26
肽泽(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many types of CPPs, and their classification is based on physical and chemical properties, sources, intake mechanisms, biomedical applications, etc., and there is no unified conclusion yet

Method used

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  • Cell-penetrating peptide as well as preparation method and application thereof
  • Cell-penetrating peptide as well as preparation method and application thereof
  • Cell-penetrating peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] By searching the human protein database, the inventors found that the C-terminus of the human SPOCK2 protein (SPARC / osteonectin, CWCV, and Kazal-like domains proteoglycan 2) is tightly bound to the cell membrane, and has multiple polysaccharide adhesion sites, and found that the human Y-like chromosome-encoded testis-specific protein gene 2 (Testis-specific protein Y encoded-like, TSPYL2) encodes a class of protein that participates in nucleosome assembly and maintains cell viability. This protein needs to be combined with DNA to function.

[0048] Further analysis found that a 17-amino acid short peptide (RRRRRKQARRPRRRRAR) at the C-terminal of the SPOCK2 protein was rich in arginine and had a strong positive charge. It was speculated that this short peptide might be a new type of human-derived membrane-penetrating peptide with Autonomous membrane-penetrating function, thus providing the human-derived cell-penetrating peptide (SPOCK2 for short) in this embodiment, which...

Embodiment 2

[0070] The only difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid is 5 times the molar amount of the resin, and the molar amount of the condensing agent 10 times the molar weight of the resin.

Embodiment 3

[0072] The difference between this example and Example 1 is that in step S2 of the method for preparing cell-penetrating peptides, the molar amount of each amino acid is 1 times the molar amount of the resin, and the molar amount of the condensing agent 10 times the molar weight of the resin.

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Abstract

The invention discloses cell-penetrating peptide as well as a preparation method and application thereof. The cell-penetrating peptide comprises a first sequence RRRRRKQARRPRRRRAR or contains a secondsequence RSSRRRRRRRRRKQRKVKR; the cell-penetrating peptide is safe and has a remarkable cell-penetrating effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a human-derived cell-penetrating peptide and its preparation method and application. Background technique [0002] Due to the barrier function of the cell membrane, it is difficult for a variety of proteins, polypeptides and oligonucleotides with therapeutic effects to enter cells to exert their medicinal effects. Therefore, how to introduce biologically active substances directly from outside the cell into the cell is a very meaningful research topic. In recent years, some small molecule polypeptides (generally no more than 30 amino acids in length) with the ability to penetrate cell membranes have been discovered one after another, which can effectively carry exogenous hydrophobic macromolecules 100 times larger than their molecular weight into cells, and have a negative effect on the host. Cells have no significant toxic side effects. These polypeptides with cell penetrating funct...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/14C07K1/04A61K47/42
CPCA61K47/42C07K7/08
Inventor 唐伟郭晓霞刘岩松段茹许力柳项
Owner 肽泽(武汉)生物科技有限公司
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