A detection method of fermented Cordyceps powder cs-4 traditional Chinese medicine decoction pieces
A technology of fermenting Cordyceps fungus powder and a detection method, which is applied in the field of medicine, can solve problems such as chromatographic column clogging, insufficient sensitivity, complex process, etc., and achieve the effects of improving extraction rate, accurate content detection results, and simple operation
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[0041] Preparation of reference solution: Accurately weigh cytidine, guanosine and adenosine reference substances, add water to dissolve and constant volume, and obtain a mixed reference solution containing cytidine, guanosine and adenosine 10-50ug per 1mL.
[0042] Selection of detection wavelength: Purified water was used as the solvent in the experiment, and the three reference substances of cytidine, guanosine and adenosine were prepared at appropriate concentrations, and UV scanning was performed. The results showed that the maximum absorption of the three reference substances were all between 250nm and 300nm. Considering comprehensively, the wavelength 260nm is selected as the experimental wavelength.
[0043] Determination method: Accurately draw reference substance solution and need testing solution respectively, inject liquid chromatograph, measure and obtain chromatogram, calculate cytidine (C 9 h 13 N 3 o 5 ), guanosine (C 10 h 13 N 5 o 5 ) and adenosine (C ...
Embodiment 1
[0049] The chromatographic conditions are: the chromatographic column is filled with octadecylsilane bonded silica gel, the column length is 15cm, the inner diameter is 2mm, the particle size is 2μm, the column temperature is 15°C, the detection wavelength is 260nm, and the phase A in the mobile phase is water , phase B is methanol, the flow rate is 0.5mL / min, the gradient elution ratio of the mobile phase is shown in Table 1, and the number of theoretical plates is not less than 3000 based on the adenosine peak.
[0050]
[0051] Table 1. Gradient elution ratio of mobile phase
[0052] Preparation of reference solution: Accurately weigh 10 mg each of cytidine, guanosine and adenosine reference substances, add 100 mL of water to dissolve to make a stock solution, take 5 mL of the stock solution into a 50 mL volumetric flask, add water to volume, and obtain 1 mL of the reference substance The solution contains 10ug each of cytidine, guanosine and adenosine.
[0053] Linear ...
Embodiment 2
[0066] The chromatographic conditions are: the chromatographic column is filled with octadecylsilane bonded silica gel, the column length is 25cm, the inner diameter is 8mm, the particle size is 10μm, the column temperature is 30°C, the detection wavelength is 260nm, and the phase A in the mobile phase is water , phase B is methanol, the flow rate is 1.0mL / min, the gradient elution ratio of the mobile phase is shown in Table 1, and the number of theoretical plates is not less than 5000 based on the adenosine peak.
[0067] Preparation of reference solution: Accurately weigh 25 mg each of cytidine, guanosine and adenosine reference substances, add 50 mL of water to dissolve to make a stock solution, take 5 mL of the stock solution into a 50 mL volumetric flask, add water to volume, and obtain each 1 mL of the reference substance The solution contains 50ug each of cytidine, guanosine and adenosine.
[0068] Linear relationship investigation: According to the above chromatographi...
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