Magnetic particle microfluidic biochip for four inflammation markers and detection method

Abstract

The invention relates to a magnetic particle microfluidic biochip for four inflammation markers and belongs to the technical field of POCT (Point-of-care Testing) detection. The biochip comprises a biosensor, wherein the biosensor comprises a wafer platform and sample application antibodies coated on the wafer platform; the sample application antibodies, a magnetic bead coupled antibody and a marker protein can form an immune complex, the concentration of the marker protein can be judged according to magnetic resistance signal intensities of the complex on the wafer platform, and the sample application antibodies are single colonial antibodies of four inflammation markers PCT, IL-6, CRP and SAA. According to the technical scheme, magnetic beads have relatively large specific surface areasand can be combined with a large number of proteins/antibodies, so that the detection sensitivity can be improved. The invention further provides a detection method with the biochip. By adopting the detection method, sensitivity, a linear range, a lower detection limit, accuracy and a specific level of a fourth generation imported chemiluminiscence method POCT product can be met, meanwhile, the product is small and exquisite in structure, convenient to carry over, good in sensitivity, accurate, short in detection time and applicable to detection in basic community hospitals.

Description

technical field [0001] The invention relates to a biochip, which is mainly used to detect four markers of inflammation in blood samples (whole blood / plasma / serum / finger blood). The invention also provides a detection method using the above biochip, which belongs to POCT detection technology field. Background technique [0002] Inflammation: The defense reaction of living tissue with vascular system to injury factors is inflammation, and vascular response is the central link in the inflammatory process. Inflammation, which is commonly referred to as "inflammation", is a defense response of the body to stimuli, manifested as redness, swelling, heat, pain and dysfunction. Inflammation can be infectious inflammation caused by infection, or non-infectious inflammation not caused by infection. Normally, inflammation is beneficial and is the body's automatic defense response, but sometimes it is also harmful, such as attacks on the body's own tissues, inflammation in transparent ...

AI Technical Summary

Problems solved by technology

[0012] The first-generation detection technology competition method (radioimmunoassay, RIA): There are many factors affected, and the RIA sample requires a large amount. Because extraction is usually required before detection, it not only increases the operation steps, but also because the recovery rate of extraction is usually Only 80% to 90%, the coefficient of variation (CV) is relatively large, which reduces the precision of detection;

[0013] The second-generation detection method non-competitive method (radioimmunoassay, RIA): immunoassay using double-antibody sandwich, no extraction, high antibody specificity, its sensitivity, precision and specificity are better than competitive methods, but time-consuming Still long and difficult to apply to automation;

However, this detection method still has the following shortcomings: optical background noise, sample photobleaching, and optical signal attenuation will interfere with the sensitivity and specificity of the detection. At the same time, the equipment cannot be miniaturized and POCT, and it is mainly operated by professionals. , unable to achieve bedside detection, ambulance detection and home detection

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