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Method for preparing varicella virus stock solution and its application

A technology of varicella virus and original solution, which is applied in the direction of virus antigen components, viruses, antiviral agents, etc., and can solve the problems of low recovery rate of original solution, excessive bovine serum residue, and impact on production

Active Publication Date: 2022-03-01
SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, washing is a key process, if the washing is not sufficient, it will directly lead to the residue of the batch of bovine serum exceeding the standard and be discarded directly
If the washing is excessive, the diseased cells will be washed away, resulting in a low recovery rate of a certain batch of stock solution and a drop in titer, which in turn affects the yield.

Method used

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  • Method for preparing varicella virus stock solution and its application
  • Method for preparing varicella virus stock solution and its application
  • Method for preparing varicella virus stock solution and its application

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Embodiment Construction

[0024] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings. The embodiments of the present invention are only used to illustrate the technical solutions of the present invention without limitation. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the invention can be modified or equivalently replaced , without departing from the spirit and scope of the technical solution of the present invention, all of which shall be covered by the claims of the present invention.

[0025] The MRC-5 cell and virus strain information that following embodiment of the present invention adopts are as follows:

[0026] The source of MRC-5 cells is Shanghai Rongsheng Biopharmaceutical Co., Ltd., which is originally from ATCC, and the working seed batch is 27 generations. The virus seed of VZV-O...

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Abstract

A method for preparing varicella virus stock solution, characterized in that 5v / v% bovine serum is added to serum-free medium at the cell culture stage of MRC-5 cells, then VZV-OKa strain is inoculated, and serum-free medium is used instead The virus was cultured as a maintenance solution to obtain a stock solution of varicella virus. The method for preparing varicella virus stock solution of the present invention adopts a suitable serum-reduced medium to cultivate cells, cultivates VZV virus under serum-free conditions, and applies it to the preparation of live attenuated varicella vaccine, which can greatly reduce the bovine serum albumin in the vaccine. Protein residues are above 60%.

Description

technical field [0001] The invention relates to a method for preparing biological products, in particular to a method for preparing varicella vaccine, which reduces the residual amount of bovine serum albumin. Background technique [0002] Bovine serum (FBS) is a mixture of complex components, the main components of which are proteins, polypeptides, hormones, and other components such as amino acids, glucose, and trace elements. Serum has extremely important functions in cell culture, such as: providing hormones that can promote cell exponential growth, nutrients that are absent or contained in basal medium, and certain low-molecular nutrients; providing binding proteins, identifying vitamins, Lipids, metal ions, and combined with toxic metals and pyrogens to detoxify; it is the source of factors required for cell adhesion and spreading; it acts as a pH buffer; and it provides protease inhibitors to make the remaining pancreas Protease inactivation protects cells from damag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/25A61P31/22
CPCA61K39/12A61P31/22C12N7/00A61K2039/5254C12N2710/16751C12N2710/16734
Inventor 朱绍荣
Owner SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD