Surface-enhanced Raman scattering nucleic acid aptamer sensor for detection of polychlorinated biphenyls and its application
A Raman scattering nucleic acid and aptamer sensor technology, applied in Raman scattering, instruments, measurement devices, etc., can solve problems such as low sensitivity and complex sample pretreatment, improve sensitivity, save sample detection time, operation simple effect
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Embodiment 1
[0031] A surface-enhanced Raman scattering nucleic acid aptamer sensing and analysis method for PCB detection, the sensor is constructed through the following steps:
[0032] (1) All glass instruments used were soaked in aqua regia, cleaned with high-purity water, and dried for later use. 100 mL 0.01% (w / w) HAuCl 4 Bring to a boil, then add 1.5 mL of 1% (w / w) sodium citrate. Stir at 100°C for 15-20min until the solution turns wine red, that is, Au NPs are synthesized. Then add 800 μL of AgNO 3The solution (0.5%, w / w) was added to the boiling 100mL AuNPs solution, and 1mL of 1% (w / w) sodium citrate solution was added dropwise under stirring, then the solution was kept boiling for 1h, the heating was turned off, and at room temperature cool down.
[0033] (2) 10 mM 4-MBA was added to Au@Ag CS NPs to make the final concentration of 4-MBA 5 μM, and then reacted for a period of time. The nucleic acid aptamer solution with a concentration of 6 μM and the PCB72 solution with a c...
Embodiment 2
[0035] In order to ensure that this sensing analysis method exhibits the optimal SERS effect, the concentration of 4-MBA, the concentration of nucleic acid aptamer and the reaction time need to be optimized. The specific steps are: take 4-MBA with a concentration range of 1 μM-8 μM for Raman intensity test to obtain the optimal 4-MBA concentration; then change the concentration of nucleic acid aptamer, and consider the nucleic acid aptamer within the concentration range of 1 μM-10 μM The optimal concentration; Finally, consider the influence of different reaction times on the Raman intensity. The results showed that the optimal concentration of 4-MBA was 5 μM, the optimal concentration of nucleic acid aptamer was 6 μM, and the optimal reaction time was 25 min.
Embodiment 3
[0037] A good sensing analysis method also needs to ensure the reproducibility of the signal and the stability of the substrate. The specific experimental steps are as follows: prepare 5 batches of mixed solutions for Raman detection, select 10 points for each batch, and the relative standard deviation is 8.82%. The experimental results show that this sensing analysis method has good signal reproducibility and can ensure The reliability of the method; the experiment also conducted a Raman test on the same batch of substrates every 10 days, and the results proved that the substrates had similar Raman effects within 60 days, showing the stability of the substrates.
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