Method for detecting a plurality of anesthetics in aquatic product through triple quadrupole gas chromatography-mass spectrometry

A triple quadrupole and GC-MS technology, which is applied in the field of product safety and testing, can solve problems such as safety concerns and panic of fish anesthetics, affecting social stability, and detrimental to the healthy development of fishery economy.

Inactive Publication Date: 2018-11-06
翟纹静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lag of the supervision system and the lack of authoritative judgment on the safety of the use of anesthetics for fishing, if it is not guided and supervised in time, it may cause more a

Method used

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  • Method for detecting a plurality of anesthetics in aquatic product through triple quadrupole gas chromatography-mass spectrometry
  • Method for detecting a plurality of anesthetics in aquatic product through triple quadrupole gas chromatography-mass spectrometry
  • Method for detecting a plurality of anesthetics in aquatic product through triple quadrupole gas chromatography-mass spectrometry

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Example Embodiment

[0034] Example 1: Method for detecting multiple anesthetics in aquatic products by triple quadrupole GC / MS

[0035] 1Materials and methods

[0036] (1) Instruments and reagents

[0037] Gas chromatography-mass spectrometer / mass spectrometer (Agilent 7890B-7000C); nitrogen blowing instrument (Organomation N-EVAP); vortex mixer (IKA); ultrapure water device Milli-Q (Milliporeco); centrifuge (Xiang instrument); Electronic balance (METTLER).

[0038] Standard products: Eugenol (Eugenol, purity 99.5%), methyl eugenol (Methyl eugenol, purity 99.5%), isoeugenol (Isoeugenol, purity 98.8%) purchased from Dr. Ehrenstorfer; Eugenol-D3 (Eugenol-D3) , Purity 98.8%) purchased from AccuStandard; 3-Aminobenzoic acid methyl (3-Aminobenzoic acid methyl, purity 98.0%) purchased from TCI; Eugenyl acetate (Eugenyl acetate, purity 98.0%), methyl isoeugenol ( Methylisoeugenol (purity 98.0%) and tricaine (MS-222, purity 97.0%) were purchased from CNW; Acetisoeugenol (Acetisoeugenol, purity 98.0%) was purch...

Example Embodiment

[0077] Example 2: Triple quadrupole GC / MS method for detecting multiple anesthetics in aquatic products

[0078] A method for detecting multiple anesthetics in aquatic products by triple quadrupole GC / MS, the method comprising the following steps:

[0079] 1) Extract and purify 7 anesthetics of eugenol, methyl eugenol, isoeugenol, eugenol acetate, methyl isoeugenol, isoeugenol acetate and tricaine from aquatic products;

[0080] 2) The sample solution to be tested after purification and extraction in step 1) is analyzed by a triple quadrupole gas-mass spectrometer.

[0081] 2. A triple quadrupole GC / MS method for detecting multiple anesthetics in aquatic products according to claim 1, characterized in that the sample extraction method in step 1) is as follows: Weigh 5.00g sample and place it in In a 50mL centrifuge tube, add 50μL internal standard / substitute 1mg / L mixed standard, add 5g anhydrous sodium sulfate, 10mL n-hexane and two ceramic homogenizers, vortex and mix for 1min, ultr...

Example Embodiment

[0087] Example 3: Method for detecting multiple anesthetics in aquatic products by triple quadrupole GC / MS

[0088] A triple quadrupole GC / MS method for detecting multiple anesthetics in aquatic products includes the following steps:

[0089] The first step: extract the anesthetic from the aquatic product and purify it to obtain the sample solution to be tested;

[0090] The second step: the sample liquid to be tested obtained in the first step is analyzed with a triple quadrupole gas-mass spectrometer.

[0091] The method of extracting anesthetics from aquatic products includes: placing the aquatic product sample in the first centrifuge tube, adding the internal standard / substitute standard first, then adding anhydrous sodium sulfate, n-hexane and ceramic homogenizer, vortexing and mixing. After ultrasonic extraction for 20 minutes, centrifuge at 8000 rpm for 6 minutes, after centrifugation, transfer the supernatant to a second centrifuge tube, then add n-hexane to the first centrifu...

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Abstract

The invention discloses a method for detecting a plurality of anesthetics in an aquatic product through triple quadrupole gas chromatography-mass spectrometry, which belongs to the technical field ofexamination. The method uses the triple quadrupole gas chromatography-mass spectrometry (GC-MS/MS) for screening, quantification and affirmation, a silica gel solid phase extraction column and HP-5MSUI capillary column are employed as analysis columns. and the method for determining 7 anesthetics such as eugenol, methyleugenol, isoeugenol, acetyl eugenol, methyl iso-eugenol, isoeugenyl acetate, and tricaine in the aquatic product is carried out under an electro-spray positive ion multi-reaction monitoring mode (MRM). Compared with a traditional apparatus analysis method, the detection methodprovided in the invention is simple and fast, the linear correlation is good, the detecting limit is as low as 2.0 [mu]g/kg, and the method has the characteristics of high sensitivity, anti-matrix interference, high accuracy and high stability.

Description

technical field [0001] The invention belongs to the technical field of product safety and detection, and in particular relates to a method for detecting multiple fish anesthetics in aquatic products by triple quadrupole gas chromatography. Background technique [0002] Fresh aquatic products are generally loved by Chinese consumers. Due to the diversity of aquatic product species distribution, trans-regional and long-term transportation of fresh aquatic products has become the norm. Therefore, it is even more important to effectively keep aquatic products alive during long-distance transportation. Anesthetics have sedative and hypnotic effects, and are an effective method to reduce casualties and avoid the decline in the freshness and quality of aquatic products, and are more and more widely used in the field of aquatic product distribution. Thereby along with the development of fishery, the application of fish anesthetics in domestic aquaculture industry becomes an inevita...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/14
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/72G01N2030/045G01N2030/062G01N2030/146
Inventor 翟纹静朱振华徐振东顾娟红
Owner 翟纹静
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