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Foliage spray solution for test tube plantlet of sugarcane and preparation method thereof

A technology of foliar spraying and test-tube seedlings, which is applied in botany equipment and methods, chemicals for biological control, animal repellants, etc., can solve the poison of test-tube seedlings, shorten the time of nursery, and affect the safety of sugarcane consumption And other issues

Active Publication Date: 2018-11-16
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

At present, there are many domestication methods of sugarcane test-tube seedlings on the market, mainly using growth-promoting liquid to spray sugarcane to achieve the purpose of improving the rooting rate and survival rate of sugarcane, for example, Chinese patent ZL201510273010. Growth-promoting solution and method for photosynthetic autotrophic rooting efficiency" and Chinese patent ZL201510273074.7 "Growth-promoting solution and method for improving photosynthetic autotrophic rooting efficiency of sugarcane test-tube seedlings" to promote sugarcane rooting by configuring a growth-promoting solution suitable for sugarcane growth Purpose, however, during the transplanting and rooting process of sugarcane test-tube plantlets, it is easy to be attacked by pathogens and insects, causing test-tube plantlets to rot and plant damage, affecting the growth and survival of sugarcane test-tube plantlets
[0003] During the cultivation process of sugarcane test-tube plantlets, due to the relatively high sugar content of the medium, they are transplanted from the culture room environment to the external natural environment. The test-tube plantlets have weak resistance and are easily attacked by molds, attracting stem borers and aphids, which will greatly reduce the sugarcane test-tube plantlets. Survival rate, growth rate, and delayed time to emerge from the nursery. At present, sugarcane diseases and insect pests are mainly controlled by applying pesticides. However, the test-tube seedlings are weak, and pesticides will cause poisoning to the test-tube seedlings. The pesticide residues in sugarcane will also affect the consumption of sugarcane. Safety. At present, there is no disclosure of any kind of promoting liquid for spraying at the rooting and transplanting stage of sugarcane test tube seedlings. It can not only promote rooting, enhance resistance, and improve survival rate, but also resist the invasion of pathogens and insect pests, promote growth, and shorten the time of emergence from the nursery. time

Method used

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  • Foliage spray solution for test tube plantlet of sugarcane and preparation method thereof
  • Foliage spray solution for test tube plantlet of sugarcane and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0023] The sugarcane test-tube plantlet foliar spray liquid of the present embodiment comprises the indolebutyric acid of 20mg / L, the naphthaleneacetic acid of 10mg / L, the sucrose of 20g / L, the L proline of 50mg / L, the biological removal agent of 300mg / L Bacteria, 2mg / L Tween and 10mg / L gibberellin.

[0024] Wherein, the biological fungicide of the present embodiment is composed of the following components by mass: 15 parts of dandelion extract, 3 parts of oregano extract, 17 parts of peppermint extract, 11 parts of Chinese toon leaf extract and 40 parts distilled water.

[0025] ① Among the biological fungicides, the preparation method of the dandelion extract is as follows: divide the dandelion powder with a water content of 3% into two parts, one part is extracted with a 90% ethanol solution by volume, and the filtrate is collected by filtration and concentrated by rotary evaporation , drying to obtain Dandelion Extract I; another part is mixed with sherwood oil at a weigh...

Embodiment 2

[0044] The sugarcane test-tube plantlet foliage spray liquid of the present embodiment comprises the indolebutyric acid of 50mg / L, the naphthaleneacetic acid of 50mg / L, the sucrose of 70g / L, the L proline of 100mg / L, the biological removal agent of 400mg / L. Bacteria, 5mg / L Tween and 20mg / L gibberellin.

[0045] Wherein, the biological fungicide of the present embodiment consists of the following components by mass: 28 parts of dandelion extract, 43 parts of oregano extract, 35 parts of peppermint extract, 41 parts of Chinese toon leaf extract and 65 parts distilled water.

[0046] ① Among the biological fungicides, the preparation method of the dandelion extract is as follows: divide the dandelion powder with a water content of 3% into two parts, one part is extracted with an ethanol solution with a volume percentage of 95%, and the filtrate is collected by filtration and concentrated by rotary evaporation , drying to obtain dandelion extract I; another part is mixed with she...

Embodiment 3

[0065] The sugarcane test-tube plantlet foliage spray liquid of the present embodiment comprises the indolebutyric acid of 30mg / L, the naphthaleneacetic acid of 30mg / L, the sucrose of 50g / L, the L proline of 70mg / L, the biological removal agent of 350mg / L. Bacteria, 3mg / L Tween and 15mg / L gibberellin.

[0066] Wherein, the biological fungicide of the present embodiment is composed of the following components by mass: 20 parts of dandelion extract, 20 parts of oregano extract, 21 parts of peppermint extract, 24 parts of Chinese toon leaf extract and 45 parts distilled water.

[0067]① Among the biological fungicides, the preparation method of the dandelion extract is as follows: divide the dandelion powder with a water content of 3% into two parts, one part is extracted with an 80% ethanol solution by volume, and the filtrate is collected by filtration and concentrated by rotary evaporation , drying to obtain dandelion extract I; another part is mixed with sherwood oil at a we...

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Abstract

The invention relates to the technical field of sugarcane planting and especially relates to a foliage spray solution for test tube plantlet of sugarcane and a preparation method thereof. The spray solution mainly contains indolebutyric acid, naphthylacetic acid, sucrose, L-proline, a bio-sterilizing agent, Tween and gibberellin. By reasonable combination of the components, the foliage spray solution can effectively promote rooting and growth of rootless test tube plantlet of sugarcane. The foliage spray solution is also characterized in that the bio-sterilizing agent mainly includes a dandelion extract, an origanum vulgare extract, a mint extract and a cedrela sinensis leaf extract, so that functions of inhibiting growth of mildews and dispelling pests are achieved. The foliage spray solution can prevent the test tube plantlet of sugarcane from pests and increase survival rate and accelerate delivery of the test tube plantlets, and is free of pesticide residue.

Description

【Technical field】 [0001] The invention relates to the technical field of sugarcane planting, in particular to a liquid for spraying sugarcane test-tube seedling leaves and a preparation method thereof. 【Background technique】 [0002] Sugarcane is a vegetatively propagated crop. At present, tissue culture is mainly used to cultivate sugarcane at home and abroad to restore its species. Sugarcane tissue culture generally goes through four stages, that is, primary culture (or establishment of a sterile culture system), subculture multiplication culture, rooting culture and seedling hardening and transplanting. At present, there are many domestication methods of sugarcane test-tube seedlings on the market, mainly using growth-promoting liquid to spray sugarcane to achieve the purpose of improving the rooting rate and survival rate of sugarcane, for example, Chinese patent ZL201510273010. Growth-promoting solution and method for photosynthetic autotrophic rooting efficiency" and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/26A01N43/38A01N37/10A01N45/00A01N43/12A01N43/16A01N43/36A01P21/00A01P17/00A01P3/00
CPCA01N37/10A01N43/12A01N43/16A01N43/36A01N43/38A01N45/00A01N65/00A01N65/26A01N65/22A01N65/12A01N65/08
Inventor 刘丽敏何为中梁阗刘红坚翁梦岺
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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