Strain for producing Songgang mycin main component griseofulvin as well as method

A technology of griseofulvin and penicillium griseofulvin, applied in the field of bioengineering, can solve the problems of limited agricultural application, high fermentation cost, and low conversion rate of sugar, and achieve the effect of reliable source of raw materials

Active Publication Date: 2018-11-20
福州工微生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] At present, griseofulvin produced by submerged fermentation in China has been industrially produced at a fermentation level of around 25,000 μg / mL for many years, and the conversion rate of saccharin is low, and the fermentation cycle exceeds 240 hours. After a period of obvious stagnation, the fermentation efficiency is still low, resulting in high fermentation costs and limited agricultural promotion and application

Method used

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  • Strain for producing Songgang mycin main component griseofulvin as well as method
  • Strain for producing Songgang mycin main component griseofulvin as well as method
  • Strain for producing Songgang mycin main component griseofulvin as well as method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the breeding of high-yield griseofulvin bacterial strain FA868

[0053] 1. Screening of griseofulvin-producing strain F3215

[0054] 1. Collection of soil samples and preliminary screening of Penicillium

[0055] (1) Soil sample collection

[0056] Generally, in fertile soil with more organic matter, the number of microorganisms is the largest. In neutral and alkaline soil, bacteria and actinomycetes are the main species. There are more molds in acidic red soil and forest soil. Orchards, vegetable gardens and wild fruit growing areas, etc. Carbohydrate-rich soils and swamps are rich in yeast and mold.

[0057]Use a sampling shovel to remove floating soil about 5cm from the surface, take 10-15g of soil samples at 5-15cm, put it into a sterilized empty test tube prepared in advance, seal it with a cotton plug, and then seal it with kraft paper. The soil in the north is dry, and samples are taken at 10-20cm. Number the bags and record the location, soil t...

Embodiment 2

[0143] Embodiment 2, fermentation application and characteristics of variant strain FA-868

[0144] 1. Effects of different carbon sources on the fermentation of the mutant strain FA868

[0145] On the basis of the strain FH1816, the effects of different carbon sources on the fermentation level of the mutant strain FA868 were further compared, and a single-factor comparative test of seven carbon sources, including lactose, glucose, sucrose, maltose syrup, wheat flour, corn flour and rice flour, was carried out. And compared with the original strain F3215, the results are shown in Table 6.

[0146] Table 6 Effects of different carbon sources on the fermentation titer of griseofulvin in mutant strain FH1816

[0147]

[0148] The results showed that the mutant strain FA868 also grew and fermented to produce griseofulvin in these nitrogen sources, but with lactose as the carbon source, the fermentation unit was the lowest among the seven carbon sources, and the order of the fe...

Embodiment 3

[0167] Embodiment 3, Songgangmycin production method and application

[0168] One, Songgangmycin production method

[0169]Utilizing the griseofulvin prepared in Example 2 to produce pinefulvin, the key technology is to realize the water solubility of the main component of pinefulvin—griseofulvin.

[0170] 1. Based on the fact that griseofulvin is water-insoluble, the basic characteristics of biopesticides are water-soluble. Previously, 4-(P-azidohydranylazino)butylamine was used as a cross-linking agent to combine with ash under UV irradiation. The flavomycin extract undergoes a carboxyl reaction, and the griseofulvin changes from water-insoluble to water-soluble.

[0171] 2. In this method, the fermented liquid after the fermentation is finished is separated from the solid and liquid, the griseofulvin is in the spore, and the mycelium is granulated and dried to form a granule, and the griseofulvin content is 200,000 μ / g , Dissolved in dimethylformamide according to 30%, st...

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Abstract

The invention discloses a strain for producing a Songgang mycin main component griseofulvin as well as a method. The invention provides penicillium griseofulvum FA868, and the preservation number in the China Center for Typical Culture Collection (CCTCC) is CCTCC NO:M 2018186. The Songgang mycin main component griseofulvin is produced by using the penicillium griseofulvum FA868; the whole processof biologically synthesizing the griseofulvin can be completed without taking lactose and core pulp as main raw materials and through simple seeds and fermentation formula of taking rice as carbon andnitrogen sources and adding a proper amount of inorganic salt; and the characteristics of a high-resistance precursor-chloride is maintained, the total amount of the chloride can reach to above 2.0 percent in the fermentation process, and the dechlorinated griseofulvin content is guaranteed to be below 0.2 percent. A reliable raw material source is provided for developing Songgang mycin serving as anti-fungal biological pesticide.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bacterial strain and a method for producing griseofulvin, the main component of pinegangmycin. Background technique [0002] Griseofulvin, as a class of antibiotics for both medical and agricultural purposes, was the first generation of anti-dermatophytic drugs developed by foreign manufacturers. It was launched in the 1960s and was once hailed by the international pharmaceutical community as a major drug against fungal diseases. Milestone drug. With the continuous advancement of biotechnology and bioinformatics, the scope of application of griseofulvin is also expanding, the most eye-catching aspect is the application and development of anticancer drugs and agriculture, which opens up the market for griseofulvin. new way. After decades of rapid development, my country has now become the only producer of griseofulvin API, and the total production capacity has expanded ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/04C12R1/80
CPCC12N1/14C12P17/04C12N1/145C12R2001/80
Inventor 吴松刚翁雪清黄钦耿陈健黄建忠施碧红谢必峰吴宇峰
Owner 福州工微生物科技有限公司
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