Autotetraploid induction and rapid propagation system as well as construction method and application thereof

Abstract

The invention belongs to the technical field of plant regeneration through a tissue culture technology, and discloses an autotetraploid induction and rapid propagation system as well as a constructionmethod and application thereof. According to the autotetraploid induction and rapid propagation system, the concentration of colchicine solution subjected to suction filtration and sterilization is 0.15 percent and the induction time is 16 hours; and autotetraploid bud seedlings are cultivated in a culture medium of MS+6-BA 1mg/L+NAA 0.2 mg/L+IBA 0.6 mg/L for 20 days. On one hand, the economic benefit of thesium chinense planting can be improved, higher-quality germplasm resources are provided for artifical culture of thesium chinense, the existing conditions of promiscuous varieties and lowquality are changed and the influence by plant diseases and insect pests is avoided; on the other hand, the environment cost of the thesium chinense planting can be reduced and damage to natural vegetation is effectively avoided; in addition, great potential in production and application of medicine materials is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and in particular relates to an autotetraploid induction and its rapid propagation system as well as its construction method and application. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Thymeceae is a perennial herb, one of the special medicinal materials in my country, mainly produced in Hunan, Anhui and other places. The rhizome is used as medicine, and modern pharmacological studies have shown that thyme grass mainly contains lactone compounds, polysaccharides and volatile oil. A large number of studies have proved that thyme has important functions such as regulating immunity, regulating gastrointestinal function, anti-aging, diuretic, dilating blood vessels, anti-inflammatory, antibacterial, sedative, and hypoglycemic. At present, there are problems such as low yield, rapid de...

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Problems solved by technology

[0004] (1) At present, there are problems such as low yield, rapid degeneration of varieties, year-by-year decline in the quality of medicinal materials, and unstable content of active ingredients in the cultivation of Chinese herbal medicines of Chinese herbal medicine.

[0005] (2) Thingrass is suitable for growing in high-altitude and shady mountainous areas, and is not suitable for continuous cropping. It needs to be continuously planted in the way of land reclamation, which will greatly damage the natural vegetation and cause damage to the ecological environment of the planting site.

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