Magnetic bead cleaning method and device

Abstract

The invention relates to the technical field of sample analysis, in particular to a method and device for washing magnetic beads. The method includes the steps that washing fluid with a first designated amount is injected into a to-be-washed reaction cup; washing fluid with a second designated amount is injected into the to-be-washed reaction cup, wherein after the washing fluid with the second designated amount is injected, the liquid level of the washing fluid in the to-be-washed reaction cup is not lower than the highest position of splashing fluid on the cup wall of the to-be-washed reaction cup; the following operation is cyclically executed N times, waste fluid in the to-be-washed reaction cup is sucked by a fluid sucking needle adopting double layers of washing needles, and washingfluid with a third designated amount is injected into the to-be-washed reaction cup by a fluid injection needle adopting double layers of washing needles, wherein the fluid sucking needle is sleeved with the fluid injection needle adopting the double layers of washing needles, a fluid injection opening of the fluid injection needle is higher than a fluid suction opening of the fluid suction needle, and N is a positive integer larger than or equal to 1; and the waste fluid in the to-be-washed reaction cup is sucked, and the magnetic beads are washed.

Description

technical field [0001] The invention relates to the technical field of sample analysis, in particular to a magnetic bead cleaning method and device. Background technique [0002] Chemical Luminescence Immunity Analyzer (Chemical Luminescence Immunity Analyzer, CLIA) is the use of chemical or bioluminescence system as an indicator system for antigen-antibody reactions, in order to quantitatively detect antigens or antibodies, a new type of ultra-luminescence analysis and immunoreaction combined to establish a new microanalytical techniques. It has both high sensitivity of luminescence analysis and high specificity of antigen-antibody reaction. Magnetic bead washing is not a reaction step in the CLIA process, but it is the key to the success of the experiment. The purpose of magnetic bead washing is to wash away the substances in the reaction solution that are not bound to the solid-phase antigen and antibody, as well as the interfering substances that are non-specifically a...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a magnetic bead cleaning method and device to solve the problem in the prior art that the reaction solution is splashed on the cuvette wall, resulting in splashing, which leads to inaccurate experimental results.

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