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Caps markers related to frost resistance in wheat seedling stage and method for identifying frost resistance in wheat seedling stage

A technology for frost resistance and wheat seedlings, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve time-consuming and other problems

Active Publication Date: 2021-07-23
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

The former requires the construction of a mapping population, which takes a long time, and the identified loci / molecular markers are limited to the differences between the two parents

Method used

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  • Caps markers related to frost resistance in wheat seedling stage and method for identifying frost resistance in wheat seedling stage
  • Caps markers related to frost resistance in wheat seedling stage and method for identifying frost resistance in wheat seedling stage
  • Caps markers related to frost resistance in wheat seedling stage and method for identifying frost resistance in wheat seedling stage

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Embodiment Construction

[0021] 1. Identification of frost resistance phenotype in wheat seedling stage

[0022] From December 2015 to February 2016, there were continuous low temperature weather in Huaibei, Anhui. The present invention carried out seedling freezing injury (Freezing tolerance, FT) identification on 403 wheat varieties planted in Huaibei Wupu Farm in three periods: December 17, 2015, January 19, 2016, and February 16, respectively. Recorded as 15FT, 16FT-1, 16FT-2. Calculate the average value of the freezing damage phenotype in the three periods, and record it as FT-M. The level records of freezing damage in wheat fields refer to the agricultural industry standards of the People's Republic of China and are divided into 5 levels:

[0023]

[0024] 2. Extraction of wheat genomic DNA by SDS-Tris saturated phenol method

[0025] (1) Take 2-3 wheat seeds and place them in a 2ml sterilized centrifuge tube, and use MP high-throughput tissue grinder to grind them into powder.

[0026] (...

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Abstract

The invention discloses a CAPS marker related to frost resistance at seedling stage of wheat and a method for identifying frost resistance at seedling stage of wheat, and relates to the technical field of molecular breeding of wheat. The CAPS marker is named CAPS-Ta-2-4. The nucleotide sequence of the CAPS marker is: SEQ ID NO.1; the method for identifying the frost resistance of wheat seedlings using the CAPS marker, the method includes step 1, extracting the genomic DNA of the wheat to be tested; step 2, using the SEQ ID NO. 2 and the sequence described in SEQ ID NO.3 carry out PCR amplification to the wheat genomic DNA obtained in step 1, and obtain the amplified product; step 3, utilize BtsaⅠ endonuclease to carry out enzyme digestion to the described amplification product, obtain enzyme digestion Product; Step 4, when the restriction product is 185bp and 70bp, the genotype of its corresponding amplified product is named as CAPS-Ta-2-4-A, and its corresponding wheat seedling stage frost resistance is strong, when the restriction product When it is 255bp, the genotype of its corresponding amplification product is called CAPS-Ta-2-4-G, and its corresponding wheat seedling stage frost resistance is weak; The CAPS mark of the present invention development can provide molecule for the genetic improvement of wheat frost resistance mark.

Description

technical field [0001] The invention relates to the technical field of wheat molecular breeding, in particular to a CAPS marker related to frost resistance at seedling stage of wheat and a method for using the marker to identify frost resistance at seedling stage of wheat. Background technique [0002] About 40% of the world's population uses wheat as a staple food, and China is the world's largest wheat producer and consumer. Therefore, high and stable yields have always been an important goal of wheat breeding (He Zhonghu et al. 2018). However, wheat suffers from low temperature and freezing damage (<-5°C) at the seedling stage, which seriously affects the high and stable yield of wheat. Breeding wheat varieties with strong frost resistance is an effective way to solve this problem. [0003] Freezing tolerance (FT) traits in wheat are jointly controlled by multiple genes. The reported frost resistance-related genes in wheat are mainly located on chromosomes 1B, 2B, 1D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张海萍卢杰常成张璨姜昊董连生邵辉司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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