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A black boletus bacterial strain and domestication method thereof

A technology of black boletus and strains, applied in the field of biological agriculture, to achieve the effects of simple and fast processing methods, stable fruiting and yield, and strong strain stability

Active Publication Date: 2021-03-05
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a black boletus bacterial strain and its domestication method to solve the defects in the existing black boletus cultivation technology

Method used

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  • A black boletus bacterial strain and domestication method thereof
  • A black boletus bacterial strain and domestication method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:

[0081] S1, cultivating fruiting bodies based on pomelo tree as host tree under low temperature environment and collecting;

[0082] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;

[0083] S3, the culture medium of the bacterial meat tissue block was cultured at 25° C. for 20 days in the dark to obtain the parent species.

[0084] S4, expanding the parent species to obtain liquid strains;

[0085] S5, inoculating the liquid bacteria in the first culture container at 26°C under dark light conditions, and culturing for 30 days;

[0086] S6, covering the mycelium with soil in the culture container, and culturing at 27° C. for 10 days to obtain the soil-covered mycelium that has entered the Cuilei stage.

[0087] S7, inoculating the soil-covered ...

Embodiment 2

[0095] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:

[0096] S1, cultivating and collecting fruiting bodies based on gardenia as a host tree in a low temperature environment;

[0097] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;

[0098] S3, the culture medium of the bacterial meat tissue pieces was cultured at 26° C. in the dark for 25 days to obtain the parent species.

[0099] S4, expanding the parent species to obtain liquid strains;

[0100] S5, inoculating the liquid bacteria in the first culture container at 27°C under dark light conditions, and culturing for 30 days;

[0101] S6, covering the mycelium with soil in the culture container, and culturing at 28° C. for 10 days to obtain the soil-covered mycelium that has entered the Cuilei stage.

[0102] S7, inoculating the soil-covered m...

Embodiment 3

[0110] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:

[0111] S1, cultivating and collecting fruiting bodies based on coffee trees as host trees in a low temperature environment;

[0112] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;

[0113] S3, the culture medium of the bacterial meat tissue block was cultured at 28° C. in the dark for 30 days to obtain the parent species.

[0114] S4, expanding the parent species to obtain liquid strains;

[0115] S5, inoculating the liquid bacteria in the first culture container at 28°C under dark light conditions, and culturing for 30 days;

[0116] S6, covering the mycelium with soil in the culture container, and culturing at 29° C. for 10 days to obtain the soil-covered mycelium that has entered the Cui Lei stage.

[0117] S7, inoculating the soil-covere...

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Abstract

The invention provides a black boletus strain and a domestication method thereof. Wherein, the black boletus strain is the black boletus strain HZ14080, and the preservation number is: CGMCC No.15271. The present invention provides a black boletus bacterial strain and a cultivation method thereof, so that the black boletus bacterial strain is more suitable for industrialized cultivation, the processing method is simple and fast, the bacterial strain has strong stability, fruiting and stable output, thereby being a black boletus. The large-scale industrial cultivation of bacteria has created convenient conditions.

Description

technical field [0001] The invention belongs to the technical field of biological agriculture, and in particular relates to a black boletus strain and a domestication method thereof. Background technique [0002] Black boletus (Phlebopus portentosus), also known as dark brown reticulate boletus, is a precious tropical facultative mycorrhizal edible fungus, distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand and my country Yunnan, Guangxi and Hainan and other places. Black boletus is fresh and tender in quality, delicious in taste, rich in nutritional value, high in protein and low in fat, rich in 17 kinds of essential amino acids and mineral elements necessary for human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. Good health food and has good medicinal value, deeply loved by consumers. [0003] Black boletus is one of the few boletus edible fungi we know that has evolved and can leave the host tree for a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
CPCA01G18/00A01G18/40
Inventor 吴开云纪开萍纪光燕罗顺珍魏学林李春会高丽霞
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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