Rapid detection method and detection kit for melatonin

Abstract

The invention discloses a rapid detection method and a detection kit for melatonin. The rapid detection method for the melatonin comprises the following subsequent steps: firstly, mixing a to-be-tested sample with an extractant, standing and taking supernatant as probational liquid; secondly, dropping the probational liquid on qualitative filter paper; after drying in air, and dropwise adding a p-dimethylaminobenzaldehyde solution at the titration position of the probational liquid; observing the color change of the filter paper; if the color of the filter paper turns blue, showing that the to-be-tested sample contains the melatonin; if the color of the filter paper has no change, showing that the to-be-tested sample does not contain the melatonin. The rapid detection method disclosed by the invention has the advantages of simple operation, short time and high efficiency; compared with methods of high performance liquid chromatography, chromatography-mass spectrometry, enzyme-linked immunity and the like, the rapid detection method has the characteristics of simplicity, fastness and no need of reference substances; high accuracy is realized, and common ingredients such as tablets,capsules, pills, granules and oral liquid have no interference on the detection of melatonin substances; the sensitivity is high and the detection limit is smaller than 0.04mg; consumables are few aswell as environmental protection and safety are achieved.

Description

technical field [0001] The invention relates to a rapid detection method and a detection kit of melatonin, belonging to the detection field of melatonin. Background technique [0002] Melatonin (MT), also known as melatonin, melatonin, and epinenaline, belongs to indole heterocyclic compounds, and its molecular formula is N-acetyl-5-methoxytryptamine. Antioxidant system, drugs that prevent oxidative damage to cells, have a sleeping effect, but the dosage needs to be controlled. Long-term or excessive use at will may interfere or cause the entire endocrine system to be disturbed and cause other diseases. [0003] The detection method of existing melatonin content mainly contains following several kinds: [0004] 1. Thin layer chromatography: [0005] After the components to be measured in the sample were extracted by methanol, the resulting solution and the reference solution were developed on a silica gel plate with a developing agent, taken out, dried in the air, and insp...

AI Technical Summary

Problems solved by technology

[0006] The advantage of thin-layer chromatography is that it does not require expensive analytical instruments. The disadvantages of this method are: low chromatographic resolution, complex components of Chinese patent medicines, many interference factors, and easy misjudgment. Environmental factors have a great influence, so it is not suitable for on-site testing, and it is not suitable for on-site testing with high mobility

The disadvantages are: the instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high; when the retention time of the chromatographic peak of the coexisting components is close to the retention time of melatonin, it is easy to cause misjudgment; the environmental requirements of the instrument High, it needs to be placed fixedly, not suitable for on-site testing; the instrument has high requirements on the professional skills of testing personnel, and is not suitable for grass-roots personnel to operate

[0014] The disadvantages of enzyme-linked immunosorbent assay are that the cost of enzyme-linked immunosorbent immunoassay kit is relatively expensive, the operation is complicated and cumbersome, and the detection time of a single sample is long, which is not suitable for screening a large number of samples