Egg granule protein with anti-breast cancer activity and its preparation method and application
An anti-breast cancer and spider spider technology, applied to peptide/protein components, medical preparations containing active ingredients, applications, etc., to achieve the effect of clear targets and strong specificity
Active Publication Date: 2021-07-30
HUNAN NORMAL UNIVERSITY
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[0008] The present invention intends to provide a new protein with anti-breast cancer activity found in the egg grains of the spider spider. At present, there is no relevant report on the protein with anti-breast cancer activity in the egg grains of the spider spider
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[0042] The invention will be further described below in conjunction with the embodiments and accompanying drawings, but they are not used to limit the scope of the invention.
[0043] Due to the limited source of E. coli eggs, the content of the anti-breast cancer active protein in eggs is not high, so it is difficult to directly extract and prepare samples from eggs. In the embodiment of the present invention, samples are prepared by heterologous expression of Escherichia coli .
[0044] 1. Cloning and heterologous expression of anti-breast cancer protein coding genes
[0045] (1) First, a series of open reading frames (ORFs) that might encode active proteins and polypeptides were obtained through the analysis of the transcriptome of E. According to the results of bioinformatics analysis, we selected one of the ORFs for gene cloning and heterologous expression, and the nucleotide sequence of the selected coding gene was as follows:
[0046]
[0047] The nucleotide sequence...
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Abstract
The present invention provides an Egg Grain Protein with anti-breast cancer activity and its preparation method and application. The amino acid sequence of the protein is shown in SEQ ID NO: 1, and its molecular weight is 10172.2 Da. The protein can be prepared by obtaining the coding gene from the transcriptome of the Escherichia coli, and then expressing it heterologously in Escherichia coli. The protein can specifically act on the human breast cancer cell line MDA-MB-231 cells, inhibit the proliferation and metastasis of MDA-MB-231 cells, and induce its apoptosis, so as to realize the anti-breast cancer effect, and can be applied to the preparation of inhibitory MDA ‑MB‑231 cell growth drugs, as precursor molecules to prepare drugs for the treatment of breast cancer, and in combination with other anti-tumor drugs to prepare drugs for the treatment of breast cancer. Proteins with anti-breast cancer activity can inhibit breast cancer cells from multiple aspects, so they can not only be used as precursor molecules for the development of breast cancer drugs, but also play a role in related studies such as tumorigenesis and development mechanisms, showing value application prospects.
Description
technical field [0001] The present invention relates to a protein with anti-breast cancer activity and its preparation method and application, especially relates to a protein capable of inhibiting the proliferation and metastasis of human breast cancer cell line MDA-MB-231 and inducing its apoptosis The egg granule protein of the spotted spider and its preparation method and application. Background technique [0002] Female breast cancer is one of the most common malignant cancers in the world. The risk of breast cancer generally increases with age, which seriously threatens women's health and even life safety. The incidence of breast cancer is increasing, which is associated with increasing life expectancy, increasing urbanization and the widespread adoption of Western lifestyles. In China, the incidence of breast cancer is 11.61 / 100,000, which is relatively high. At the same time, due to the accurate screening, diagnosis, intervention and effective treatment of breast ca...
Claims
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IPC IPC(8): C07K14/435C12N15/12C12N15/70A61K38/17A61P35/00A61P15/14
CPCA61K38/00A61P15/14A61P35/00C07K14/43518C12N15/70
Inventor 王贤纯徐德宏
Owner HUNAN NORMAL UNIVERSITY