Rapid automatic focusing method for biological microscope
An automatic focus and microscope technology, applied in the field of medical inspection, can solve the problems of slow focusing speed, poor picture quality, narrow focus surface, etc., and achieve the effect of saving time, improving speed and precision, and improving quality
Active Publication Date: 2018-12-18
SHENZHEN REETOO BIOTECHNOLOGY CO LTD
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[0002] At present, the existing automatic focusing methods of biological microscopes have slow focusing speed and narrow focusing surface (after the glass slide finds the focus on the Z axis, it is easy to lose focus after moving to the X and Y axes in a large range), the consequences of this It is a waste of a lot of time in the focusing process, and after the focusing is completed, the clarity of the picture collection around the focus point is completely dependent on the horizontality of the clamping slide platform. In places with a large edge slope, the quality of the collected pictures is extremely high. Difference
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[0034] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.
[0035] Such as figure 1 As shown, the present invention discloses a fast autofocus method of a biological microscope, and the fast autofocus method includes the following steps:
[0036] Step S1. During the process of controlling the Z-axis motor of the biological microscope to drive the stage up, at least two or more pictures are obtained from the microscope inspection area of the reagent card placed on the stage through the sensor installed on the biological microscope, specifically Generally speaking, it is only necessary to ensure that the highest point of the moving stage does not touch the objective lens of the biological microscope; The total moving distance is generally 500um; before performing step S1, the light source, objective lens, and X, Y, and Z axis motors of the stage can be initialized.
[0037] Step S2. Calculate the obt...
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The invention discloses a rapid automatic focusing method for a biological microscope. The method comprises the following steps of S1, in a process of controlling a Z-axis motor of the biological microscope to drive an objective table to move up, obtaining at least two or more pictures for a microscopic examination area of a reagent card placed on the objective table through a sensor; S2, performing calculation on the obtained pictures separately to obtain definition values corresponding to the pictures, and then performing sorting sequentially to obtain a first definition value sequence; S3,finding out a sorting position corresponding to the picture with the maximum definition value in the first definition value sequence and a sorting position corresponding to the last definition value in the first definition value sequence, and performing calculation to obtain a first distance for which the corresponding objective table near a focal point of the biological microscope moves; and S4,according to the first distance, controlling the Z-axis motor to drive the objective table to move down, thereby enabling the objective table to be located near the focal point of the biological microscope. Compared with the prior art, the time is greatly saved.
Description
technical field [0001] The invention relates to a medical detection technology, in particular to a fast automatic focusing method of a biological microscope. Background technique [0002] At present, the existing automatic focusing methods of biological microscopes have slow focusing speed and narrow focusing surface (after the glass slide finds the focus on the Z axis, it is easy to lose focus after moving to the X and Y axes in a large range), the consequences of this It is a waste of a lot of time in the focusing process, and after the focusing is completed, the clarity of the picture collection around the focus point is completely dependent on the horizontality of the clamping slide platform. In places with a large edge slope, the quality of the collected pictures is extremely high. Difference. Contents of the invention [0003] The purpose of the present invention is to provide a fast automatic focusing method for a biological microscope, and the technical problem to...
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Login to View More IPC IPC(8): G02B21/24
CPCG02B21/241
Inventor 李乔亮陈齐文吴亚杰唐洪浩余志刚何旭东齐素文
Owner SHENZHEN REETOO BIOTECHNOLOGY CO LTD