Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for preventing apple tissue culture browning and adventitious bud induction

A bud induction, apple technology, applied in the field of plant tissue culture, can solve defects, apple tissue culture browning and other problems, achieve the effect of reducing browning rate, low production cost, and good technical advantages

Active Publication Date: 2021-10-19
HENAN AGRICULTURAL UNIVERSITY
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this application is to provide a combined medium for apple tissue culture and the corresponding apple tissue culture method, so as to alleviate and overcome the serious browning defect in the existing apple tissue culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for preventing apple tissue culture browning and adventitious bud induction
  • A method for preventing apple tissue culture browning and adventitious bud induction
  • A method for preventing apple tissue culture browning and adventitious bud induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Taking the construction of the virus-free seedling tissue culture system of the columnar apple Runtai No. 1 as an example, the method for reducing the browning rate of apple tissue culture in this application is introduced as follows.

[0034] (1) Select explants and perform pretreatment

[0035] Take the columnar apple Runtai No. 1 as an example. From March to May, before 15:00 on a sunny day (this time is for the Henan region, other regions can be adjusted according to the growth habits of apples), select young shoots that are free from diseases and insect pests and grow robustly. As an explant (length 8-10cm, thickness 0.6-8cm); put the explant in a fruit bag (15×18cm) that is yellow on the outside and black on the inside for shading treatment. When bagging, first open the paper bag by hand , make the paper bag bulge, then put about 10cm long young shoots drawn in the same year into the bag, then shrink the mouth of the bag to the branches, and tie it with a sealing ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for preventing apple tissue culture browning and adventitious bud induction. The combination medium includes primary tissue culture medium and adventitious bud induction medium. The apple tissue culture method comprises the steps of selecting explants and pretreatment, inducing culture of the first generation, inducing the formation of adventitious buds and the like. In this application, aiming at the serious browning problem in the existing apple tissue culture, the optimization and exploration of the technical scheme was carried out through various methods such as explant material extraction, explant pretreatment, disinfection time, medium composition, hormone ratio, etc., and finally Effectively prevent the oxidation of phenolic substances, and better prevent the occurrence of browning of apples. Statistics show that the browning rate can be reduced to below 25%, which solves the problem of high browning rate of apple tissue culture, and shows good The technical advantages have also laid a good technical foundation for improving the rapid reproduction efficiency of apples.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for preventing apple tissue culture browning and adventitious bud induction. Background technique [0002] Apple is one of the main cultivated fruit tree species in my country. Its area and output both rank first in the world. The development of the apple industry is of great significance to increasing farmers' income and adjusting the industrial structure. [0003] There are many varieties and types of apples, among which the columnar apple is a special dwarf mutation type, with short internodes, few lateral branches, and an upright single-stem shape. It is considered to be the most ideal for ultra-high-density cultivation and intensive production of apples. plant type. [0004] Among the columnar apples, "Runtai No. 1" is a columnar apple variety that has been cross-bred for many years. It has been widely promoted in my country due to its good...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 白团辉谢贝阳郑先波黄松宋春晖张玉冯建灿
Owner HENAN AGRICULTURAL UNIVERSITY