Preparation method of collagen

A collagen and mass ratio technology, applied in the field of biotechnology, can solve the problem of low protein purity, and achieve the effect of improving the purity and strengthening the effect of enzymatic hydrolysis

Inactive Publication Date: 2018-12-21
FOSHAN SENANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology uses different techniques such as grinding or tumbling to break down hard-to-reach materials like fish scale fibers into smaller pieces that are easier to remove from food products made therefrom without damaging them. By adding certain chemical agents (like acids) at specific points along this processing path, these small particles help dissolve any remaining material found within the fish' s body while also removing unwanted substances called phytate. These processes improve the quality and yield of animal feed ingredients produced from seafood waste.

Problems solved by technology

This patents describes how different types of proteins found naturally occur within various parts (flesh) of seafood like mollusks). These natural compounds help keep them flexible during water movements without losing their function due to being surrounded by soluble components that make up about 75% of all material on earth. Additionally, these ingredients contain numerous mineral nutrients essential for cell metabolism including metal salts, carboxylates, glutathione redox cycling agents, polyunsaturated oxygen radicals, hormonal regulators, etc., making them valuable sources for cosmetic products.

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0023] The preparation method of the immobilized enzyme is as follows: polyacrylamide, water and activated carbon are mixed according to the mass ratio of 1:20:4-6, then the mixed enzyme is added, stirred, genipin with 2-4% of the activated carbon mass is added, stirred, and static place, and freeze-dry to obtain the immobilized enzyme;

[0024] The mixed enzyme is formed by mixing pepsin and trypsin at a mass ratio of 1:1-3; the amount of mixed enzyme added is 10-15% of the mass of activated carbon;

[0025] A preparation method of collagen, the preparation method comprising the steps of:

[0026] (1) Dry the fish scales at 50°C for 20 minutes, crush them at 500r / min for 10 minutes, collect the crushed matter, put the crushed matter and mixed solvent in a ball mill at a mass ratio of 4 to 6:2, and perform ball milling at 600r / min for 15 minutes. Collect ball mills;

[0027] (2) Put the ball mill into a steam explosion device for steam explosion. The condition of steam explo...

Embodiment 1

[0031] The mixed solvent is formed by mixing ethanol and acetone in a volume ratio of 5:3;

[0032] The mixed acid is prepared by mixing citric acid, humic acid and water in a mass ratio of 6:2:18;

[0033] The washing solvent is ethylene glycol;

[0034] The preparation method of the immobilized enzyme is: mix polyacrylamide, water and activated carbon according to the mass ratio of 1:20:6, then add the mixed enzyme, stir, add genipin with 4% active carbon mass, stir, stand still, freeze-dry , to obtain immobilized enzyme;

[0035] The mixed enzyme is a mixture of pepsin and trypsin at a mass ratio of 1:3; the amount of mixed enzyme added is 15% of the mass of activated carbon;

[0036] A preparation method of collagen, the preparation method comprising the steps of:

[0037] (1) Dry fish scales at 50°C for 20 minutes, pulverize at 500r / min for 10min, collect the pulverized product, put the pulverized product and mixed solvent in a ball mill at a mass ratio of 6:2, perform...

Embodiment 2

[0042] The mixed solvent is formed by mixing ethanol and acetone in a volume ratio of 5:2;

[0043] The mixed acid is prepared by mixing citric acid, humic acid and water in a mass ratio of 5:2:17;

[0044] The washing solvent is 1.2mol / L ethanol solution;

[0045] The preparation method of the immobilized enzyme is as follows: mix polyacrylamide, water and activated carbon in a mass ratio of 1:20:5, then add the mixed enzyme, stir, add genipin with 3% active carbon mass, stir, stand still, freeze-dry , to obtain immobilized enzyme;

[0046] The mixed enzyme is formed by mixing pepsin and trypsin at a mass ratio of 1:2; the added amount of the mixed enzyme is 13% of the mass of activated carbon;

[0047] A preparation method of collagen, the preparation method comprising the steps of:

[0048](1) Dry fish scales at 50°C for 20 minutes, pulverize at 500r / min for 10min, collect the pulverized material, put the pulverized material and mixed solvent in a ball mill at 600r / min f...

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PUM

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Abstract

The invention relates to a preparation method of collagen, which belongs to the technical field of biotechnology. The preparation method comprises the following steps: destructing an internal structure of fish scales in a ball milling manner, adding a mixing solvent in a ball milling process, softening hydroxyapatite inside the fish scales by virtue of the mixing solvent, then performing the steamexplosion treatment to destruct a fiber ply structure inside the fish scales, enabling a hydroxyapatite layer to be ruptured, then performing the treatment by using mixed acid, wherein the mixed acidcan effectively enter the fish scales to eliminate the hydroxyapatite, the humic acid in the mixed acid can further adsorb and remove the free calcium, then performing the mixed enzymolysis for the fish scales and immobilized enzyme, so that since the active carbon is used as a carrier, the impurity absorption and discoloration can be effectively performed, the enzymolysis effect can be further improved, the purity of the collagen can be improved, and an effect for extraction and purification can be further achieved by virtue of the bacterial effect.

Description

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Claims

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Application Information

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Owner FOSHAN SENANG BIO TECH CO LTD
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