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Preparation method and application of octacosanol-containing lipidosome

A technology of dioctadecanol and taurine dioctadecanol ester, applied in the field of bioengineering, can solve the problems of poor water solubility of higher aliphatic alcohols, affecting flavor and sensory appearance, etc., and achieves good uniformity, is conducive to absorption and The effect of circulation in the body

Active Publication Date: 2018-12-25
深圳市承泽信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the poor water solubility of higher fatty alcohols, adding them to beverages will affect their flavor and sensory perception

Method used

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  • Preparation method and application of octacosanol-containing lipidosome

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1) Mix 5ml of an aqueous solution containing 1.5wt% polyvinyl alcohol (purchased from Aladdin Reagent Company, product number P119363) with 5ml of dichloromethane, and make a water-dichloromethane emulsion under high-speed stirring at 8000r / min;

[0026] 2) Add 66mg of octacosanol and 20mg of taurine to the prepared water-dichloromethane emulsion, add 500mg of sulfatase (the specific activity of the enzyme is 5000U / mg protein), and react at 37°C for 6h , the reaction is over, the stratification is allowed to stand, and the lower layer is octacosyl taurine solution (TO solution);

[0027] 3) Add 40mg of cholesterol to 2ml of water to prepare cholesterol aqueous solution, then mix it with TO solution, perform ultrasonic emulsification with 250W power, emulsify for 20min, remove dichloromethane by rotary evaporation at room temperature, then add 0.6ml 30g / L citric acid aqueous solution and mix well , hydrated for 3 hours, and then used a liposome extruder to make TO liposo...

Embodiment 2

[0029] 1) Mix 10ml of an aqueous solution containing 1.0wt% polyvinyl alcohol (purchased from Aladdin Reagent Company, product number P119363) with 5ml of dichloromethane, and prepare a water-dichloromethane emulsion under high-speed stirring at 10000r / min;

[0030] 2) Add 60 mg octacosanol and 15 mg taurine to the prepared water-dichloromethane emulsion, add 300 mg sulfatase (the specific activity of the enzyme is 1000 U / mg protein), and react at 30 ° C for 4 h , the reaction is over, the stratification is allowed to stand, and the lower layer is octacosyl taurine solution (TO solution);

[0031] 3) Add 25mg of cholesterol to 2.5ml of water to prepare a cholesterol aqueous solution, then mix it with TO solution, perform ultrasonic emulsification with 200W power, emulsify for 30min, remove dichloromethane by rotary evaporation at room temperature, then add 0.5ml of 20g / L citric acid aqueous solution to mix Homogenize, hydrate for 2 hours, then use a liposome extruder to make T...

Embodiment 3

[0033] 1) Mix 5ml of an aqueous solution containing 2.0wt% polyvinyl alcohol (purchased from Aladdin Reagent Company, product number P119363) with 5ml of dichloromethane, and prepare a water-dichloromethane emulsion under high-speed stirring at 9000r / min;

[0034] 2) Add 75mg of octacosanol and 25mg of taurine to the prepared water-dichloromethane emulsion, add 400mg of sulfatase (the specific activity of the enzyme is 4000U / mg protein), and react at 40°C for 8h , the reaction is over, the stratification is allowed to stand, and the lower layer is octacosyl taurine solution (TO solution);

[0035] 3) Add 50 mg of cholesterol to 1.25 ml of water to prepare an aqueous solution of cholesterol, then mix it with TO solution, perform ultrasonic emulsification with 300 W power, emulsify for 10 min, and remove dichloromethane by rotary evaporation at room temperature, then add 0.625 ml of 40 g / L citric acid aqueous solution to mix Homogenize, hydrate for 4 hours, and then use a liposo...

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Abstract

The invention discloses a preparation method of octacosanol-containing lipidosome, including the steps of adding octacosanol and taurine into water-dichloromethane emulsion and performing catalytic synthesis to obtain taurine-octacosanol ester solution; adding cholesterol solution into the taurine-octacosanol ester solution obtained in the first step, performing ultrasonic emulsification, performing rotary evaporation at room temperature to remove dichloromethane to obtain lipidosome solution; hydrating the lipidosome solution, extruding to obtain octacosanol-containing lipidosome with uniformgrain size through a lipidosome extruder, and finally freezing and drying into powder to obtain the finished octacosanol-containing lipidosome product. Since the octacosanol-containing lipidosome isprepared by synthesis of octacosanol and taurine, the problem of poor water solubility of higher aliphatic alcohol is solved, the effect of colourless and odourless is achieved when added to foods, cosmetics and beverages, and the lipidosome has good homogeneity. Besides, octacosanol and taurine have complementary and synergistic effect to the organism and are good for absorption and in-vivo circulation.

Description

technical field [0001] The invention relates to a preparation method and application of octacosanol-containing liposomes, belonging to the field of bioengineering. Background technique [0002] Taurine, also known as taurine, is a sulfur-containing non-protein amino acid, and its chemical name is 2-aminoethanesulfonic acid. It is a sulfur-containing amino acid with various physiological functions in the human body. It is one of the most important amino acids in the human body for brain development, vision enhancement, anti-inflammation, antipyretic, lowering blood pressure, lowering blood sugar, strengthening liver and gallbladder. Taurine has no toxic and side effects, so developed countries attach great importance to its research and application. In recent years, with the in-depth research on its physiological effects and nutritional value, its application has become very extensive. Taurine is widely used as nutritional health products and food additives abroad. According...

Claims

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Application Information

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IPC IPC(8): A61K8/46A61K8/34A61K8/14A61Q19/00A23L33/10A23L33/175
CPCA23V2002/00A61K8/14A61K8/342A61K8/466A61K2800/10A61K2800/592A61Q19/00A23L33/10A23L33/175A23V2200/30A23V2250/0644
Inventor 汤佳鹏葛彦朱俐
Owner 深圳市承泽信息科技有限公司
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