Fermentation method and application of a kind of bacillus

A technology of bacillus and fermentation method, applied in the field of microorganisms, can solve the problems of underutilization of active ingredients, waste of resources, environmental pollution, etc.

Active Publication Date: 2022-03-11
SHANGHAI FONDIN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main treatment methods of these wastes are to dry them as feed or discharge them directly. The active ingredients are not fully utilized, which not only causes great pollution to the environment, but also causes a waste of resources.

Method used

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  • Fermentation method and application of a kind of bacillus
  • Fermentation method and application of a kind of bacillus
  • Fermentation method and application of a kind of bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A fermentation method for bacillus, specifically comprising the steps of:

[0033] Inoculate the bacillus in the culture medium, and culture it on a shaking table at 25° C. and pH 6.5-7.5 for 45 hours. After the fermentation is completed, obtain the bacillus fermentation liquid, and spray-dry the bacillus fermentation liquid to obtain the bacillus spore powder; The shaking table speed is 180r / min.

[0034] The medium includes the following materials in parts by weight: 150 parts of yeast fermentation waste, 6 parts of sodium chloride, 3 parts of corn flour, 0.5 parts of manganese sulfate monohydrate, and 750 parts of water.

[0035] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 20 parts of urea, 30 parts of nitrogen and potassium compound fertilizer, 25 parts of ammonium dihydrogen phosphate, 400 parts of seaweed extract, humic 500 parts of Potassium Acid, 100 parts of the abov...

Embodiment 2

[0037] A fermentation method for bacillus, specifically comprising the steps of:

[0038] Bacillus was inoculated into the culture medium, and cultured on a shaker at 30° C. and pH 6.5-7.5 for 50 hours. After the fermentation was completed, a Bacillus fermentation liquid was obtained; the shaker speed was 160 r / min.

[0039] The medium includes the following materials in parts by weight: 300 parts of yeast fermentation waste, 4 parts of sodium chloride, 6 parts of corn flour, 0.3 parts of manganese sulfate monohydrate, and 800 parts of water.

[0040] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 30 parts of urea, 20 parts of nitrogen and potassium compound fertilizer, 20 parts of ammonium dihydrogen phosphate, 500 parts of seaweed extract, humic 550 parts of Potassium Acid, 150 parts of the above-mentioned bacillus spore powder that makes.

Embodiment 3

[0042] A fermentation method for bacillus, specifically comprising the steps of:

[0043] Bacillus was inoculated into the culture medium, and cultured on a shaker at 35° C. and pH 6.5-7.5 for 48 hours. After the fermentation was completed, a Bacillus fermentation broth was obtained; the shaker rotated at 120 r / min.

[0044] The medium includes the following materials in parts by weight: 230 parts of yeast fermentation waste, 3 parts of sodium chloride, 5 parts of corn flour, 0.4 parts of manganese sulfate monohydrate, and 700 parts of water.

[0045] A kind of bio-organic fertilizer containing the bacillus spore powder prepared above comprises the following materials in parts by weight: 25 parts of urea, 28 parts of nitrogen and potassium compound fertilizer, 30 parts of ammonium dihydrogen phosphate, 600 parts of seaweed extract, humic 600 parts of Potassium Acid, 200 parts of above-mentioned bacillus spore powders that make.

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Abstract

The invention relates to a fermentation method of bacillus. The medium used in the fermentation process includes the following materials in parts by weight: 150-300 parts of yeast fermentation waste, 3-6 parts of sodium chloride, and 3 parts of corn flour Part-6 parts, 0.3-0.5 parts of manganese sulfate monohydrate, 700-800 parts of water. The invention also relates to the application of the bacillus spore powder prepared by the method. The fermented bacillus fermentation broth is made into bacillus spore powder and then compounded with other plant nutrients, such as seaweed extract, and finally compounded into bio-organic fertilizer, which not only greatly improves the soil aggregate structure, but also improves the microbial environment of the soil , effectively prevent soil diseases, reduce the amount of pesticides; and can reduce the input of chemical fertilizers in my country, significantly increase the content of soil organic matter and improve soil biological activity, which plays a very important role in improving soil fertility, crop yield and quality, and enhancing crop resistance. .

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a fermentation method of bacillus and an application thereof. Background technique [0002] Yeast is a single-celled fungus that can survive in both aerobic and anaerobic environments and is a facultative anaerobic bacteria. Yeast is the earliest single-cell microorganism used by human beings and the largest number, which can be used for brewing production. Yeasts are widely distributed in nature, and most yeasts can be isolated from sugar-rich environments, such as some fruits (grapes, apples, peaches, etc.) or plant secretions (such as cactus juice) or sugar cane. The raw materials used for fermentation are usually mainly starch, molasses or other agricultural and sideline products, and the reaction can be carried out only by adding a small amount of organic and inorganic nitrogen sources. [0003] In yeast production, sugarcane molasses is used as the ferm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/80C12R1/07
CPCC12N1/20C05B7/00C05G3/00C05G3/80C05C9/00C05F11/00C05F11/02C05F11/08C05G1/00
Inventor 项金霞张忠池王保娜丁辉吴迪范佳千骆亚陈巍张建强
Owner SHANGHAI FONDIN BIO TECH
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