Rapid detection method of residual quantity of aquacide and paraquat in food

A detection method, Diquat's technology, is applied in the direction of measuring devices, instruments, scientific instruments, etc. It can solve the problems of unfriendly people and the environment, time-consuming, labor-intensive and cost-consuming problems, and achieve detection limit satisfaction, detection efficiency improvement, Quick Qualitative Effects

Inactive Publication Date: 2018-12-28
烟台杰科检测服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection method of plant food is mainly based on the industry standard "SN/T 0293-2014 Determination of paraquat and diquat residues in food of plant origin for export by liquid chromatography-mass spectrometry/mass spectrometry", which does not involve animal food In the detection method of paraquat and diquat, this method uses hydrochloric acid, wh

Method used

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  • Rapid detection method of residual quantity of aquacide and paraquat in food
  • Rapid detection method of residual quantity of aquacide and paraquat in food
  • Rapid detection method of residual quantity of aquacide and paraquat in food

Examples

Experimental program
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Example Embodiment

[0029] Example 1: Take the above-mentioned spinach sample to detect the residual content of diquat and paraquat, the steps are as follows:

[0030] (1) Extraction: Weigh 5.0 g of the uniformly prepared spinach sample into a 50 mL centrifuge tube, add 10.0 mL of a mixed solution of formic acid, methanol and water (the mixing volume ratio is 1:40:60), vortex and mix thoroughly Centrifuge at 4000 r / min for 5 min, transfer the supernatant to a 25 mL plastic colorimetric tube, and then use 10.0 mL of a mixed solution of formic acid, methanol and water (mixed volume ratio 1:40:60) for the residue. Vortex to mix, centrifuge at 4000 r / min for 5 min, combine the two extracts, and dilute to 25.0 mL with a mixed solution of formic acid, methanol and water, mix well and wait for purification.

[0031] (2) Purification: Pass 5.0 mL of the extract obtained in step (1) through a hydrophilic lipophilic copolymer solid phase extraction column, purify, and collect directly. The solid phase extractio...

Example Embodiment

[0046] Example 2: Take the above-mentioned apple samples to detect the residual content of diquat and paraquat, the steps are as follows:

[0047] (1) Extraction: Weigh 5.0 g of a uniformly prepared apple sample into a 50 mL centrifuge tube, add 10.0 mL of a mixed solution of formic acid, methanol and water (the mixing volume ratio is 1:40:60), vortex and mix thoroughly Centrifuge at 4000 r / min for 5 min, transfer the supernatant to a 25 mL plastic colorimetric tube, and then use 10.0 mL of a mixed solution of formic acid, methanol and water (mixing volume ratio 1:40:60) for the residue. Vortex to mix, centrifuge at 4000 r / min for 5 minutes, combine the two extracts, and dilute to 25.0 mL with a mixed solution of formic acid, methanol and water, mix well and wait for purification.

[0048] (2) Purification: Pass 5.0 mL of the extract obtained in step (1) through a hydrophilic lipophilic copolymer solid phase extraction column, purify, and collect directly. The solid phase extractio...

Example Embodiment

[0063] Example 3: Take the above-mentioned pork sample to detect the residual content of diquat and paraquat, the steps are as follows:

[0064] (1) Extraction: Weigh 5.0 g of a uniformly prepared pork sample into a 50 mL centrifuge tube, add 10.0 mL of a mixed solution of formic acid, methanol and water (the mixing volume ratio is 1:40:60), vortex and mix thoroughly Centrifuge at 4000 r / min for 5 min, transfer the supernatant to a 25 mL plastic colorimetric tube, and then use 10.0 mL of a mixed solution of formic acid, methanol and water (mixing volume ratio 1:40:60) for the residue. Vortex to mix, centrifuge at 4000 r / min for 5 min, combine the two extracts, and dilute to 25.0 mL with a mixed solution of formic acid, methanol and water, mix well and wait for purification.

[0065] (2) Purification: Pass 5.0 mL of the extract obtained in step (1) through a hydrophilic lipophilic copolymer solid phase extraction column, purify, and collect directly. The solid phase extraction colum...

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Abstract

The invention discloses a rapid detection method of residual quantity of aquacide and paraquat in food, and specifically relates to a detection method for detecting the residual quantity of aquacide and paraquat in food via ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry based on a hydrophilic interaction chromatography HILIC mode. The method comprises the stepsof extraction, purification, membrane filtration and tandem triple quadrupole mass spectrometry detection. The method disclosed by the invention has the advantages of having simple operation and goodreproducibility, effectively removing the impurities in a sample extracting solution, being able to perform qualitative and quantitative analysis on the aquacide and paraquat in food at the same timequickly, completely meeting the technical requirements of China and the European Union, the United States and Japan for safety detection, and providing a forceful technical support for ensuring the food safety of our people and the healthy development of foreign export trade.

Description

Technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a rapid detection method for diquat and paraquat residues in food. Background technique [0002] Paraquat, whose chemical name is 1,1'-dimethyl-4,4' bipyridine cation salt, is a fast-killing herbicide with contact and certain systemic effects. It can be quickly absorbed by plant green tissues, causing it to die. No effect on non-green organizations. In the soil, it quickly combines with the soil and becomes passivated. It is ineffective to plant roots, perennial underground stems and ratoons. Paraquat is extremely toxic to humans and has no specific antidote. The mortality rate of oral poisoning can reach more than 90%. It has been banned or strictly restricted by more than 20 countries. Since July 1, 2014, my country has revoked the registration and production license of paraquat water, and stopped production; however, it retains the registration of the parent d...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/726G01N2030/062
Inventor 冷蕾池连学张禧庆马金秀郭海霞尉玮王丹赵煜炜
Owner 烟台杰科检测服务有限公司
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