A kind of method for rapid propagation and seedling growth of flue-cured tobacco

A technology for rapid seedling breeding and tissue culture, which is applied in the field of tobacco production to achieve the effects of prospering the rural economy, increasing the yield of tobacco leaves, and reducing the incidence of insect pests

Active Publication Date: 2021-03-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology and medium formula for using the rapid propagation technology for the production of flue-cured tobacco seedlings have not yet been found, which is very much needed in practical applications.

Method used

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  • A kind of method for rapid propagation and seedling growth of flue-cured tobacco
  • A kind of method for rapid propagation and seedling growth of flue-cured tobacco
  • A kind of method for rapid propagation and seedling growth of flue-cured tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Tobacco Seedling Acquisition

[0031] Wrap the seeds of tobacco variety K326 (provided by Guangdong Tobacco Qingyuan City Co., Ltd.) with gauze, rinse them in running water for 10 to 15 minutes, and then soak the seeds in 75% ethanol solution for 30 seconds on an ultra-clean workbench. Rinse 3 times; then disinfect in 0.1% HgCl solution for 5-7 minutes; rinse 5 times with sterile water; sow in 1 / 2MS medium. Germination culture was carried out under (26±1)°C and 16h / d light conditions. Then subculture, in the subculture medium: MS+0.5mg / L naphthaleneacetic acid (NAA)+1.0mg / L6-benzylaminopurine (6-BA)+7.5~8.0g / L kara powder+0.05g / Differentiate buds on L-inositol + 30g / L sucrose; then root and strengthen seedling medium B: 1 / 2MS + 7.5-8.0g / L kara powder + 0.2mg / LNAA + 0.05g / L inositol + 30g / L of sucrose+0.5g / L activated carbon (AC) to take root and grow strong seedlings into required aseptic seedlings.

Embodiment 2

[0032] Induction of embodiment 2 callus

[0033] Take aseptic seedlings with a leaf age of about 3, cut the leaves into 0.5cm*0.5cm size, sow them in the 16 kinds of media in the following table 1, and connect 8 bottles for three replicates for each treatment, and inoculate 5 pieces in each bottle , cultured at (26±1)°C, dark conditions until callus appeared, and then moved to (26±1)°C, 16h / d light conditions for cultivation. Observe and record every five days, and study the callus morphology, growth speed, and induction rate. The specific results are shown in Table 1.

[0034] Table 1 Effects of different hormone concentration ratios on callus growth and induction rate

[0035]

[0036] As can be seen from the data in Table 1, under the culture conditions of the present invention, the medium selection formula for inducing callus production is MS+Kara powder 7.5~8.0g / L+inositol 0.05g / L+sucrose 30g / L+0.2 mg / L naphthaleneacetic acid (NAA) + 2.0mg / L kinetin (KT), the callus ...

Embodiment 3

[0037] The differentiation subculture of embodiment 3 buds

[0038] During the bud differentiation, the 4 formulations with the best effect in the callus induction were taken, and then the shoots were subcultured. Each treatment received 8 bottles for three replicates, and each bottle was inoculated with 4 evenly cut shoots.

[0039] The bud differentiation subculture formula adopts several formulas that perform better in callus induction. The results of different concentrations of hormone ratios on bud differentiation are as follows:

[0040] From Table 2 and figure 1 It can be seen that among the various formulas tested, the value-added rate of A3 buds has reached 100%, the pollution rate is low, and the effect of differentiation and subculture is good. The buds of A3 are in the best shape, and the buds are relatively clustered. Long roots, long seedlings.

[0041] Table 2 Effects of different hormone concentration ratios on bud differentiation value-added rate and bud poi...

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Abstract

The invention discloses a method of flue-cured tobacco tissue culture fast breeding seedling culture, and relates to the technical field of tobacco production. The method comprises the steps of callustissue induction, bud differentiation subculture, rooting and seedling strengthening and the like. The flue-cured tobacco tissue culture fast breeding study work is systematically performed; the seedling domestication time being 3 to 5 days is needed in a process from a tissue culture chamber to provisonal planting; in addition, the provisonal planting time needs to be properly advanced; the proper provisonal planting leaf age is 3 to 4 leaf age; after the provisonal planting for about 45 days, the culture and seedling strengthening effects can be achieved. In the tissue culture seedling field growth period, the flower and leaf disease incidence rate is reduced by 80.04 percent through being compared with a conventional seedling; the insect pest occurrence rate is reduced by 52.73 percent; the medium and upper grade tobacco proportion is increased by 12.58 percent; the tobacco leaf yield is increased by 8.36 percent; the output value is increased by 17.41 percent; the income of the tobacco farmers is increased through the flue-cured tobacco fast breeding seedling technology; the rural economy is improved; good social benefits are shown.

Description

technical field [0001] The invention relates to the technical field of tobacco production, in particular to a method for tissue culture and rapid propagation of flue-cured tobacco seedlings. Background technique [0002] Tissue culture is a means of rapid plant reproduction, and it is also an ideal way for plant improvement, germplasm preservation and secondary biomass production. A large number of virus-free or virus-free plants can be used for continuous operation and annual trial production, saving land and space. [0003] Tobacco is an important economic crop in my country. At present, sexual reproduction is generally used in tobacco production in my country, but the propagated tobacco seedlings are uneven, prone to mutation, and easy to be infected with germs, resulting in frequent diseases and quality degradation during growth and development, which not only reduces the availability of tobacco leaves, It also affects the economic income of tobacco farmers. At the sam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓世媛陈建军王维许冬梅李淮源常娟娟
Owner SOUTH CHINA AGRI UNIV
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