A kind of isolated deciduous teeth preservation solution and preparation method thereof

A technology for preserving liquid and deciduous teeth, which is applied in the field of cell engineering, which can solve problems such as pollution and easy damage to microorganisms, and achieve the effects of long-term storage, avoiding microbial contamination, and strong protection

Active Publication Date: 2021-11-16
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a preservation solution for deciduous teeth in vitro, which can effectively solve the problems of easy damage and microbial contamination when deciduous teeth are preserved in vitro

Method used

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  • A kind of isolated deciduous teeth preservation solution and preparation method thereof
  • A kind of isolated deciduous teeth preservation solution and preparation method thereof
  • A kind of isolated deciduous teeth preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Deciduous teeth from one kind of preservation solution containing glucose 1g per 100mL in the preservation solution, glutathione 0.05g, 3 mL of human serum albumin, amino acids 0.5g, Ve 1g, trehalose 0.2g, 12 mL thermosensitive hydrogel, penicillin 0.5g, streptomycin 0.3g, amphotericin B 0.02g, solute remaining medium is a pH 7.4 phosphate buffer.

[0038] Wherein the method for preparing thermosensitive hydrogel of: mixing a polyacrylamide, methacrylic acid, acrylic acid and chitosan, followed by the addition of ammonium persulfate, stir and let stand 15H reaction, the reaction product was washed three times, to obtain a temperature sensitive hydrogel rubber; wherein the weight polyacrylamide, methacrylic acid, acrylic acid, ammonium persulfate and chitosan ratio of 8: 1: 0.6: 0.15: 1.

[0039] The preservation solution is prepared as follows:

[0040] Under sterile conditions, each of the above components were uniformly mixed to prepare preservation solution obtained from...

Embodiment 2

[0044] An isolated cream storage liquid containing 0.5 g of glucose per 100 ml, human blood albumin 1 mL, reduced glutathione 0.05 g, amino acid 0.5 g, VE 0.5g, trehalose 0.3g, temperature sensitive water gel 20 ml, penicillin 0.6 g, streptomycin 0.5 g, amphotericin B 0.05 g, and the remaining solute medium is a phosphate buffer having a pH of 7.6.

[0045] Among them, the preparation method of the temperature sensitive water gel is: mixed polyacrylamide, methacrylic acid, acrylic acid, and chitosan, then add sodium sulfate, stir well, and then reactive for 15 h, washed 3 reaction products, and temperature-sensitive hydrocoagulation Glue; wherein the polyacrylamide, methacrylic acid, acrylic acid, chitosan, and sodium sulfate are 10: 0.5: 0.8: 0.2: 0.6.

[0046] The preparation method of the preservation fluid is:

[0047] Under sterility conditions, the above components were mixed uniform, and the exclusive milk storage liquid was prepared.

[0048] The method of preserving the d...

Embodiment 3

[0051] An externally decodable water storage liquid containing glucose 1.2g, reduced glutathione 0.04 g, human blood albumin, 0.3 g of amino acid, 0.1g, temperature sensitive hydrocarnics, 10ml, penicillin 0.2 g, streptomycin 0.1 g, amphotericin B 0.01 g, the remaining solute medium is a phosphate buffer having a pH of 7.2.

[0052] Among them, the preparation method of the temperature sensitive water gel is: mixing polyacrylamide, methacrylic acid, acrylic acid, and chitosan, then adding the moonloyloyloyoyl, stirred well, after standing for 15 h, washing 3 times reaction product, to be temperature-sensitive water Gel; wherein the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan, and oxidation of lauryloylum is 6: 0.8: 1: 0.3: 1.

[0053] The preparation method of the preservation fluid is:

[0054] Under sterility conditions, the above components were mixed uniform, and the exclusive milk storage liquid was prepared.

[0055] The method of preserving the ...

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Abstract

The invention discloses an isolated deciduous tooth preservation solution and a preparation method thereof. The preservation solution includes the following components in parts by weight: 0.5-1.2 parts of glucose, 0.01-0.05 parts of reduced glutathione, 0.1-0.5 parts of amino acids, 1-5 parts of human serum albumin, 0-1 part of Ve, seaweed 0.1-0.3 parts of sugar, 10-20 parts of temperature-sensitive hydrogel, 0.3-1.5 parts of antibacterial drugs, and 70-90 parts of phosphate buffer solution; the preparation method is: under sterile conditions, mix the components according to the formula, Preservation solution of isolated deciduous teeth was prepared. The preservation solution prepared by the method of the invention can not only provide nutrients for the pulp tissue of the deciduous teeth in vitro, but also effectively reduce the mechanical damage in the pulp transportation process, and effectively prolong the preservation time.

Description

Technical field [0001] The present invention belongs to the technical field of cell engineering, particularly to a method of deciduous preservation and preparation in vitro. Background technique [0002] Stem cells (stem cell) is a type of pluripotent cells capable of autonomous replication (self-renewing), with the deepening of stem cell research, more and more different sources of stem cells become a hot topic of research institutions, such as bone marrow source mesenchymal stem cells, placenta derived mesenchymal stem cells, pulp derived mesenchymal stem cells, adipose-derived mesenchymal stem cells. [0003] In 2003, Miura and other separation shedding deciduous teeth pulp 7-8 year-old children in highly monoclonal ability, the ability of proliferation, in vivo pluripotent stem cells such as bone, and named SHED. [0004] Deciduous dental pulp stem cells (stem cells from human exfoliated decid-uous teeth, SHED) is between early ectodermal tissue between dental pulp mesenchyma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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