Eye cream capable of removing dark eye circles and preparation method of such eye cream
A technology for eye circles and eye cream, which is applied in the field of eye cream for removing dark circles and its preparation, can solve problems such as eye skin damage, difficult dark circles and bags under the eyes, side effects, etc., to avoid side effects, low production cost, and strong antioxidant capacity Effect
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Embodiment 1
[0035] Preparation of Bitter Melon Seed Extract
[0036] 1) Grinding 20g of bitter melon seeds into powder, adding 51.4mL concentration of 0.3mol / L sodium dihydrogen sulfate solution and 8.6mL concentration of 0.2mol / L disodium tetrapentahydrogensulfate solution to obtain mixed solution A, and then Adjust the pH value of the mixed solution A to 6, let it stand for 30 minutes, ultrasonically treat it for 3 minutes at a frequency of 53 Hz, and then centrifuge at a speed of 15000 r / min for 30 minutes at a temperature of 2 ° C to obtain a supernatant B;
[0037] 2) Adjust the pH value of the supernatant B to 9, let it stand for 30 minutes, centrifuge at a speed of 15,000 r / min for 30 minutes at a temperature of 2°C to obtain a precipitate C, and obtain a crude protein D after drying;
[0038] 3) Dissolve 7g of crude protein D in 1000mL of deionized water, then add 0.35g of beeswax, and bathe in water for 30min at a temperature of 70°C to obtain a mixed solution E;
[0039] 4) Adj...
Embodiment 2
[0041] Preparation of Bitter Melon Seed Extract
[0042] 1) Grind 20g of balsam pear seeds into powder, add 70mL of 0.3mol / L sodium dihydrogensulfate solution and 10mL of 0.2mol / L tetrapentahydrogen disodium solution to obtain mixed solution A, and then mix Adjust the pH value of solution A to 7, let it stand for 20 minutes, sonicate for 2 minutes at a frequency of 53 Hz, and then centrifuge at a speed of 15000 r / min for 20 minutes at a temperature of 6 ° C to obtain supernatant B;
[0043] 2) Adjust the pH value of the supernatant B to 9.5, let it stand for 15 minutes, centrifuge at a speed of 15,000 r / min for 20 minutes at a temperature of 6°C to obtain a precipitate C, and obtain a crude protein D after drying;
[0044] 3) Dissolve 7g of crude protein D in 1000mL of deionized water, then add 0.56g of beeswax, and bathe in water at 90°C for 15 minutes to obtain mixed solution E;
[0045] 4) Adjust the pH value of the mixed solution E to 9.0, add 30 g of alkaline protease, and...
Embodiment 3
[0047] Preparation of Bitter Melon Seed Extract
[0048] 1) Grind 20g of balsam pear seeds into powder, add 61mL of 0.3mol / L sodium dihydrogensulfate solution and 9mL of 0.2mol / L tetrapentahydrogen disodium solution to obtain mixed solution A, and then mix Adjust the pH value of solution A to 6.8, let it stand for 25 minutes, ultrasonicate for 1 minute at a frequency of 53 Hz, and then centrifuge at a speed of 15000 r / min for 25 minutes at a temperature of 4 ° C to obtain supernatant B;
[0049] 2) Adjust the pH value of the supernatant B to 9.3, let it stand for 20 minutes, and centrifuge at a speed of 15,000 r / min for 25 minutes at a temperature of 4°C to obtain a precipitate C, which is dried to obtain a crude protein D;
[0050] 3) Dissolve 7g of crude protein D in 1000mL of deionized water, then add 0.42g of beeswax, and bathe in water for 20min at a temperature of 80°C to obtain a mixed solution E;
[0051] 4) Adjust the pH value of the mixed solution E to 8.0, add 25g ...
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