Semi-natural nanometer vesicae, preparation method and application in preparing drugs for treating liver diseases

A technology of nanovesicles and drugs, applied in the fields of biochemical equipment and methods, drug combination, capsule delivery, etc., can solve problems such as low toxicity, and achieve the effect of promoting proliferation and regeneration, good liver regeneration, and simple and reliable method.

Inactive Publication Date: 2019-01-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main method to repair liver damage and promote liver regeneration is through chemical drugs ...

Method used

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  • Semi-natural nanometer vesicae, preparation method and application in preparing drugs for treating liver diseases
  • Semi-natural nanometer vesicae, preparation method and application in preparing drugs for treating liver diseases
  • Semi-natural nanometer vesicae, preparation method and application in preparing drugs for treating liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 semi-natural nanovesicle

[0025] 1. Method

[0026] 1. Preparation of primary hepatocytes

[0027] Liver primary cells were perfused according to N. Sakai et al. (N. Sakai, H.L. Van Sweringen, R.C. Quillin, R. Schuster, J. Blanchard, J.M. Burns, A.D. Tevar, M.J. 2012,56,1468-1478.) for preparation and separation. Hepatocytes were cultured in DMEM medium (Gibco, Gaithersburg, MD) containing 10% fetal bovine serum (FBS), 100 U / ml penicillin and 100 μg / ml streptomycin at 37°C, 5% CO 2 in the incubator.

[0028] 2. Preparation of semi-natural nanovesicles

[0029] Adherent primary hepatocytes were detached by scraping and resuspended in PBS. The cell suspension (5×10 6cells / ml) were sequentially extruded 5 times through 10 μm, 5 μm and 1 μm polycarbonate membranes (Nuclepore, Whatman, Inc., Clifton, NJ), respectively. For density gradient ultracentrifugation, 50% iodixanol (1ml, Axis-Shield PoC AS, Oslo, Norway), 10% iodixanol (...

Embodiment 2

[0035] Example 2 Semi-natural nanovesicles promote the proliferation of mouse primary liver parenchymal cells and promote the regeneration and recovery of partially resected mouse livers

[0036] 1. Method

[0037] 1. Proliferation promotion experiment of mouse primary liver parenchymal cells

[0038] The proliferation of mouse primary stem cells was detected by MTT assay. Hepatocytes were seeded on 96-well plates at a concentration of 5 × 103 cells / well and treated with or without semi-native nanovesicles for 24 or 48 hours. MTT solution (5 mg / ml, 20 μL / well) was added to the 96-well plate. After 4 hours of incubation, 150 μL of DMSO was added to dissolve the insoluble crystals. Absorbance values ​​were measured at 490 nm by a spectrophotometer. All experiments were performed in triplicate.

[0039] 2. Experiment on the promotion of regenerative recovery in mice with partially resected liver

[0040] Male C57Bl / 6 mice were purchased from Model Animal Research Ce...

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Abstract

The invention relates to the field of biopharmacy, in particular to semi-natural nanometer vesicae, a preparation method and application in preparing drugs for treating liver diseases. The invention discloses the semi-natural nanometer vesicae, the preparation method and the application in preparing the drugs for treating the liver diseases. The preparation method of the semi-natural nanometer vesicae is provided. Through pharmacology experiments, it is shown that the semi-natural nanometer vesicae have the effects of treating the acute and chronic liver diseases and liver damage caused by theacute and chronic liver diseases, and have a value of developing the drugs for treating the acute and chronic liver diseases and liver damage caused by the acute and chronic liver diseases. The semi-natural nanometer vesicae further have the effect of promoting regeneration and repair of part of the liver subjected to excision, and have the value of developing drugs for promoting regeneration ofthe liver which is subjected to surgical excision.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a semi-natural nanovesicle, a preparation method and an application thereof. Background technique [0002] The liver will start a self-repair process after experiencing various acute and chronic injuries. One of the most important aspects of this repair process is to replenish the necrotic liver cells due to injury through the cell regeneration mechanism. This process is also called liver regeneration. This regenerative ability of the liver largely determines whether the liver can survive acute and chronic injuries and restore its basic functions. If the liver cannot survive acute and chronic injuries and restore its basic functions through processes such as regeneration, the liver will enter acute and chronic liver failure, liver fibrosis, and cirrhosis; if it can survive acute and chronic injuries through regeneration and other processes, it will restore its basic functions. ...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K38/45A61K47/46A61P1/16
CPCA61K9/5176A61K38/45C12Y207/01091
Inventor 吴俊华吴俊艺江春平王忠夏强光辉屈振纪安来马丁张广
Owner NANJING UNIV
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