Preparation method and application of pyracantha fortuneana polysaccharide-iron complex
A technology of Pyracantha polysaccharide and complex, which is applied in the field of biomedicine, can solve the problems of weak biological activity and low iron content of polysaccharide-iron complex, and achieves the effects of optimizing process conditions, strong biological activity and increasing iron content.
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[0050] In order to solve the problem that the iron content of the polysaccharide-iron complex prepared in the prior art is low, the present invention proposes a preparation method of the pyracantha polysaccharide-iron complex, which uses the pyracantha polysaccharide as the glycoside and iron trichloride The reaction prepared Pyracantha polysaccharide iron complex, through the optimization of process conditions, increased the iron content of Pyracantha polysaccharide iron complex. At the same time, because Pyracantha polysaccharide itself has a variety of biological activities, the combination with metal ions can not only provide the body with The safe and non-toxic trace elements also exert the medicinal value of Pyracantha polysaccharide itself, and have stronger biological activity. In one embodiment of the preparation method of the pyracantha polysaccharide-iron complex provided by the present invention, the preparation method of the pyracantha polysaccharide-iron complex c...
Embodiment
[0079] (1) Extraction of Pyracantha polysaccharide
[0080] ①Take 1 kg of fresh Acanthopanax fruit, put it in a vacuum drying oven with a temperature set at 50°C, and dry it to a constant weight. Collect the extract and solid slag separately for 2 hours; dry the solid slag, then add water and extract by heating at 60°C for 3 hours, and collect the extract; combine the extracts of the two extractions to obtain the extract of Acanthopanax ;
[0081] ②Concentrate the Pyracantha extract in a water bath, then concentrate it under reduced pressure and let it stand overnight, discard the resulting precipitate, then remove the protein in the Pyracantha extract by the Sevage method, and remove it by macroporous resin adsorption. Pigment in the Acanthopanax extract to obtain the treated extract;
[0082] ③Adding 95% ethanol solution to the treated extract to make the volume concentration of ethanol reach 80%, then let stand overnight, separate the precipitate precipitated in the solut...
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