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Multi-wavelength spectrophotometric assay method for measuring contents of hydrogen peroxide on basis of Fenton oxidation and ABTS development

A technology of hydrogen peroxide and spectrophotometry, which is applied in the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problem of high measurement results and low measurement results In order to achieve the effect of rapid determination of hydrogen peroxide content, reliable results, and easy operation due to problems such as poor accuracy and high determination cost

Inactive Publication Date: 2019-01-04
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Titration methods mainly include iodometric method and potassium permanganate method. This method has high sensitivity and accurate measurement results, but there are also shortcomings such as complicated operation and many interference factors.
Chromatography has the advantages of strong specificity, high accuracy, and strong anti-interference ability, such as patent ZL 201310745895.7. However, chromatography requires the use of expensive chromatographs, resulting in high cost of determination
Chemiluminescence method has the advantages of high sensitivity, low detection limit, and accurate measurement results, but it also has disadvantages such as long measurement time and high measurement cost.
Ultraviolet-visible spectrophotometry has reliable results, fast measurement speed, and relatively simple operation, such as patents ZL 201310745891.9 and ZL 201410034574.0, but there are also shortcomings such as long reaction time, toxic reaction reagents and easy to cause environmental pollution. The test paper colorimetric method It has the advantages of simple operation and fast measurement speed, such as patent ZL201310337923.1, but there are also disadvantages such as high cost of test paper preparation, poor accuracy of measurement results, and low sensitivity
The measurement result of amperometric method is accurate and reliable, and the sensitivity is high, such as patent ZL200780034519.X, but there are also shortcomings such as high measurement results, long measurement time, and poor anti-interference ability

Method used

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  • Multi-wavelength spectrophotometric assay method for measuring contents of hydrogen peroxide on basis of Fenton oxidation and ABTS development
  • Multi-wavelength spectrophotometric assay method for measuring contents of hydrogen peroxide on basis of Fenton oxidation and ABTS development
  • Multi-wavelength spectrophotometric assay method for measuring contents of hydrogen peroxide on basis of Fenton oxidation and ABTS development

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Embodiment 1

[0028] A kind of multi-wavelength spectrophotometry based on Fenton oxidation ABTS color development of the present embodiment to measure hydrogen peroxide content comprises the following steps:

[0029] Step (1): Make a standard curve:

[0030] 1. Add 0.25mL of ferrous sulfate solution and 0.5mL of 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) sulfate mother liquor to 1.5mL of acidic purified water, Obtain the first mixed solution, then measure the absorbance value of the first mixed solution at 415nrn, and zero;

[0031] 2. Add 0.25mL hydrogen peroxide standard solution with different concentration gradients to the first mixed solution to form the second mixed solution, with a total reaction volume of 2.5mL;

[0032] 3. After reacting at room temperature for 1 min, measure the absorbance value of the second mixed solution at 415 nm after the reaction, and denote it as b;

[0033] 4. Take b as the abscissa and the final concentration of hydrogen peroxide in...

Embodiment 2

[0048] A kind of multi-wavelength spectrophotometry based on Fenton oxidation ABTS color development of the present embodiment to measure hydrogen peroxide content comprises the following steps:

[0049] Step (1): Make a standard curve:

[0050] 1. Add 0.25mL of ferrous sulfate solution and 0.5mL of ABTS sulfate mother liquor into 1.5mL of acidic pure water to obtain the first mixed solution, then measure the absorbance value of the first mixed solution at 650nm, and zero;

[0051] 2. Add 0.25mL hydrogen peroxide standard solution with different concentration gradients to the first mixed solution to form the second mixed solution, with a total reaction volume of 2.5mL;

[0052] 3. After reacting at room temperature for 0.5 min, measure the absorbance value of the second mixed solution at 650 nm after the reaction, and denote it as b;

[0053] 4. Take b as the abscissa and the final concentration of hydrogen peroxide in the second mixed solution as the ordinate to establish a ...

Embodiment 3

[0067] A kind of multi-wavelength spectrophotometry based on Fenton oxidation ABTS color development of the present embodiment to measure hydrogen peroxide content comprises the following steps:

[0068] Step (1): Make a standard curve:

[0069] 1. Add 0.25mL of ferrous sulfate solution and 0.5mL of ABTS sulfate mother liquor into 1.7mL of acidic pure water to obtain the first mixed solution, then measure the absorbance value of the first mixed solution at 732nm, and zero;

[0070] 2. Add 0.05mL hydrogen peroxide standard solution with different concentration gradients to the first mixed solution to form the second mixed solution, with a total reaction volume of 2.5mL;

[0071] 3. After reacting at room temperature for 1 min, measure the absorbance value of the second mixed solution at 732nm after the reaction, denoted as b;

[0072] 4. Take b as the abscissa and the final concentration of hydrogen peroxide in the second mixed solution as the ordinate to establish a standard ...

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Abstract

The invention discloses a multi-wavelength spectrophotometric assay method for measuring contents of hydrogen peroxide on basis of Fenton oxidation and ABTS development. The method comprises the following steps of: (1) establishing a standard curve and a linear regression equation thereof; and (2) measuring content of hydrogen peroxide in a sample. According to the multi-wavelength spectrophotometric assay method, ferrous ions are taken as an activator, and 2'-azino-bis-3-ethylbenzthiazoline-6-sulphonate (ABTS) is taken as an indicator, so that contents of hydrogen peroxide in solution can berapidly detected. According to the method, expensive instrument equipment and reagents are not required, and the measurement requirements can be satisfied through only one common ultraviolet and visible spectrophotometer, so that the measurement cost is reduced, and the method can be widely applied in the industries of environment protection, daily chemical and medical treatment so as to carry outrapid, simple and correct detection on contents of hydrogen peroxide in various oxidized, bleached or disinfected samples.

Description

technical field [0001] The invention belongs to the technical field of hydrogen peroxide detection, and in particular relates to a multi-wavelength spectrophotometric detection method for measuring hydrogen peroxide content based on Fenton oxidation ABTS color development. Background technique [0002] Due to its strong oxidizing ability and environmental protection features, hydrogen peroxide is widely used as oxidant, disinfectant, sterilant and bleaching agent in environmental protection, daily chemical, food, medical and printing and dyeing industries. In addition, hydrogen peroxide can also be activated by transition metal ions, metal oxides, microwave radiation, and ultraviolet radiation to generate hydroxyl radicals with stronger oxidizing ability (E 0 =1.8-2.7V), is widely used in the field of environmental protection, especially in the field of water treatment to remove refractory organic pollutants and inactivate pathogenic microorganisms. In the process of using ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 邹景王梦云王黛瑶马红芳苑宝玲
Owner HUAQIAO UNIVERSITY
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