Rapid propagation method of calathea insignis
A technology of arrowroot and arrow feather, which is applied in the field of rapid propagation of arrow feather arrowroot, and can solve problems such as low reproduction coefficient, slow production speed, and uneven plants
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Embodiment 1
[0034] Embodiment 1 A kind of rapid propagation method of arrow feather arrowroot
[0035] (1) Explant preparation and disinfection:
[0036] S11. Spray 3000-fold carbendazim every 7 days before seed collection on arrowhead explants, control water according to the growth environment, control water for 6 days before seed collection, and cut lateral buds according to the standard;
[0037] S12. Wipe the side buds with 75% alcohol, peel off a layer of bracts, cut off 0.5cm from the top of the side buds, and cut off 0.08cm from the bottom of the side buds, so that the side buds remain at a length of 3cm. After completion, put them in the sterilized In bottles, each bottle contains 1 / 2 volume of explants;
[0038] S13. Then add 75% alcohol to sterilize for 50 seconds, rinse twice with sterile water, and then transfer to 0.1% HgCl with 2 drops of Tween 80 2 Continue to sterilize, the sterilization time is 30min, pour out the disinfectant, pour in sterile water and wash 5 times.
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