Simple trichoderma-straw microbial fertilizer preparation method

A technology of straw and bacterial fertilizer is applied in the field of preparation of Trichoderma-straw bacterial fertilizer, which can solve the problems of high price of bacterial fertilizer products, difficulty in popularization and use, and high environmental requirements, and achieve the effects of low cost, unlimited scale and low investment.

Inactive Publication Date: 2019-01-08
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past, biological bacterial fertilizer fermentation technology required large-scale fermentation equipment, which required high environmental requirements for bacterial fertilizer fermentation. The fermentation process required professionals to operate, resulting in high costs, and the high price of bacterial fertilizer products made it difficult to promote and use

Method used

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  • Simple trichoderma-straw microbial fertilizer preparation method
  • Simple trichoderma-straw microbial fertilizer preparation method
  • Simple trichoderma-straw microbial fertilizer preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Isolation, screening, morphology and molecular identification of Trichoderma strains

[0032] (1) Isolation and screening of Trichoderma

[0033] Soil samples for isolation of Trichoderma strains were obtained from Fuzhou Forest Park, Fujian Province. The specific separation method is: weigh 3g of soil sample, add 27ml of sterile water, shake at 150r / min for 20min, let it stand for 5min, and take the supernatant as 1×10 -1 Diluent, dilute to 1×10 by gradient concentration method -2 , 1×10 -3 , 1×10 -4 . 100 μl of each gradient dilution was spread on Bengal Red separation medium. Culture at a constant temperature of 28°C for 4-5 days, pick and purify representative single colonies according to the characteristics of colony morphology, mycelium richness and sporulation production (the medium used for purification is PDA medium), after purification, -70°C, 30 % glycerol is stored for future use.

[0034] (2) Morphological identification of Trichoderma

[0...

Embodiment 2

[0041] Embodiment 2: Preparation of Trichoderma-stalk bacterial fertilizer

[0042] Preparation of Trichoderma spore suspension

[0043] Activation on the flat plate in step (2) of the above-mentioned embodiment 1 (activation step: on the Trichoderma flat plate cultivated for 2 days, use a hole puncher with a diameter of 30mm to get a bacterium cake on the outer edge of the colony, and inoculate it on the PDA flat plate in the center of the Trichoderma incubator at 28°C until green spores covered the entire plate, and stored at 4°C for later use.) Trichoderma spores were scraped off and mixed with sterile water to prepare a spore suspension with a spore concentration of 5×10 7 cells / mL, stored at 4°C, and inoculated for later use. Put the prepared spore suspension into a liquid strain fermenter with a capacity of 120L to ferment for 20 days, control the ventilation rate to 1vvm, and complete the preparation of the primary seed, with an inoculum size of 300ml.

[0044] The f...

Embodiment 3

[0050] Embodiment 3: greenhouse fertilizer effect experiment

[0051] Applying the fermented bacterial fertilizer to wheat, cucumber, and bitter melon in the greenhouse has obvious effects in promoting growth (as shown in Table 1, Table 2, and Table 3). The specific methods are as follows:

[0052] Trichoderma-straw bacterial fertilizer on wheat, cucumber, and bitter melon growth promotion analysis experiments were carried out in the greenhouse of the School of Life Science and Agronomy, Zhoukou Normal University. The test crops were wheat, which was planted as Dunyu No. 8; cucumbers, which were planted as Ludiwang cucumber ; Bitter melon, planted as the king of beautiful crowns and green swords. Set up the control group of the treatment group, the treatment group is the high-efficiency Trichoderma-stalk bacterial fertilizer developed by the present invention, the control group is without adding bacterial fertilizer (CK), the treatment group is adding Trichoderma-stalk bacteri...

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Abstract

The invention relates to a simple trichoderma-straw microbial fertilizer preparation method. An active component of trichoderma-straw microbial fertilizer is a thallus of a trichoderma asperellum strain T-A158. The preparation method comprises the following preparation steps: inoculating spore suspension of the trichoderma asperellum strain T-A158 into a culture medium of a fermentation tank for fermentation to complete preparation of first-level seeds; mixing the first-level seeds with straw powder for fermentation, wherein if the surface of a fermented material is full of spores, the preparation of second-level seeds is completed; uniformly mixing the second-level seeds, the straw powder and humic acid for fermentation till the surface is full of spores, wherein the number of effective viable bacteria in a fermentation product is greater than or equal to 0.21 x 10<8> to 0.37 x 10<8> p / g, the infectious microbe rate is less than or equal to 12 to 15 percent, and the moisture content is controlled to 20 to 30 percent. By taking the waste straws (corns, rice, wheat, rapes and the like) as the fermentation raw material to prepare the trichoderma-straw efficient microbial fertilizer,the method is low in cost, easy to operate and applicable to industrial production. The simple trichoderma-straw microbial fertilizer preparation method can prevent plant soil borne diseases, enhancethe stress resistance and the disease resistance of a plant, promote growth of the plant, improve the crop quality, effectively improve the soil environment and protect the environment.

Description

technical field [0001] The present invention relates to a kind of simple preparation method of Trichoderma-stalk bacterial fertilizer, specifically Trichoderma aculeatus ( Trichoderma asperellum ) - a preparation method of straw bacterial fertilizer. Background technique [0002] In my country, about 800 million tons of waste straw are produced every year due to agricultural production. For the convenience of farming, farmers directly burn a large amount of waste straw in the open air. The straw burned in the open air accounts for more than 30% of the total recyclable straw. In recent years, the country and the government have invested a lot of manpower and material resources to solve the problem of straw burning. The burning phenomenon has indeed been controlled, but it still cannot solve the problem fundamentally. . Due to insufficient technological transformation, the huge economic value of straw itself is difficult to play. [0003] Trichoderma ( Trichoderm spp.)...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05F17/00C05G3/80
CPCC05F11/00C05F17/00C05G3/80C05F11/08Y02W30/40
Inventor 张福丽杨同文李俐俐
Owner ZHOUKOU NORMAL UNIV
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