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A preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and application in cell labeling

A technology of nano-diamond and nano-diamond powder, which is applied in the field of preparation of monodisperse aminated nano-diamond colloid solution, can solve the problems of not being able to obtain monodisperse nano-diamond colloid, achieve high yield, low production cost, and cheap and easy-to-obtain reagents Effect

Active Publication Date: 2020-08-11
SHANGHAI BRIGHT STAR BIO-PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to propose a method for preparing a monodisperse aminated nano-diamond colloid solution and its secondary method based on the introduction of impurities that still exist in the direct amination process of the nano-diamond surface and the technical problem that monodisperse nano-diamond colloids cannot be obtained. Dispersion process and application in cell labeling, the method of the present invention can make the nanodiamond surface simple amination modification, and the obtained monodisperse colloidal solution can disperse again

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  • A preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and application in cell labeling
  • A preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and application in cell labeling
  • A preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and application in cell labeling

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Embodiment 1

[0046] A kind of preparation method of monodisperse aminated nano-diamond colloidal solution of the present embodiment, described method comprises the steps:

[0047] (1) Take ball milling beads (mass ratio of agate bead diameter 6.8mm to small bead diameter 4.2mm 1:5) and put it into a ball mill jar with a volume of 100ml, add 0.5g of the above-mentioned purified nano-diamond and 2.5g of chlorine Sodium chloride was added without adding ammonium chloride, the ball mill (model: QM-1SP2, Nanjing University Instrument Factory) was set at a linear speed of 512 m / min, the ball milling time was 2 hours, the ball mill was turned off, and the ball mill jar was removed after 30 minutes.

[0048] (2) Take out the ball milling beads in the ball milling tank, wash with deionized water (250-300ml, the volume of water can be increased appropriately), and disperse under the action of ultrasound (8000W ultrasound generally does not exceed 1min, 360W ultrasound generally 15min), Centrifuge at...

Embodiment 2

[0053] A kind of preparation method of monodisperse aminated nano-diamond colloidal solution of the present embodiment, described method comprises the steps:

[0054] (1) Take ball milling beads (mass ratio of agate bead diameter 6.8mm to small bead diameter 4.2mm 1:5) and put it into a ball mill jar with a volume of 100ml, add 0.5g of the above-mentioned purified nano-diamond and 2.5g of chlorine Ammonium chloride, 2.5g sodium chloride, set the linear speed of the ball mill (model: QM-1SP2, Nanjing University Instrument Factory) to 512m / min, the ball milling time to 2h, turn off the ball mill, and remove the ball mill jar after 30min.

[0055] (2) Take out the ball milling beads in the ball milling tank, wash with deionized water (250-300ml, the volume of water can be increased appropriately), and disperse under the action of ultrasound (8000W ultrasound generally does not exceed 1min, 360W ultrasound generally 15min), Centrifuge at a centrifugal speed of 10000r / min, set the ...

Embodiment 3

[0064] A kind of preparation method of monodisperse aminated nano-diamond colloidal solution of the present embodiment, described method comprises the steps:

[0065] (1) Take ball milling beads (mass ratio of agate bead diameter 6.8mm to small bead diameter 4.2mm 1:5) into a ball mill jar with a volume of 100ml, add 0.5g of the above-mentioned purified nano-diamond and 5g of chloride Ammonium, 2.5g sodium chloride, set the speed of the ball mill (model: QM-1SP2, Nanjing University Instrument Factory) to 512r / min, the ball milling time to 2h, turn off the ball mill, and remove the ball mill jar after 30min.

[0066] (2) Take out the ball milling beads in the ball milling tank, wash with deionized water (250-300ml, the volume of water can be increased appropriately), and disperse under the action of ultrasound (8000W ultrasound generally does not exceed 1min, 360W ultrasound generally 15min), Centrifuge at a centrifugal speed of 10000r / min, set the time for 5min, and repeat the...

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Abstract

The invention relates to a preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and application in cell labeling, belonging to the technical field of nano-diamond processing. The preparation process of the monodisperse aminated nano-diamond colloidal solution of the present invention includes: mixing the purified nano-diamond powder with ammonium chloride and sodium chloride and performing ball milling, washing the ball-milled mixture with deionized water, ultrasonication, and centrifugal preparation. have to. Secondary dispersion process: After the aminated nano-diamond colloid solution is dried, it is re-dispersed in solvents such as DMSO, water, ethanol or DMF under the action of ultrasound or shearing. The yield of the aminated nano-diamond prepared by the method of the invention is high, and the prepared aminated nano-diamond colloid has monodispersity. In addition, the method of the invention is simple and easy to operate, does not require special reaction equipment, and the raw materials are cheap and easy to obtain, the production cost is low, and the industrial production is easy, and the prepared aminated nano-diamond can be applied to cell labeling.

Description

technical field [0001] The invention belongs to the technical field of nano-diamond processing, and in particular relates to a preparation method of a monodisperse aminated nano-diamond colloidal solution and its secondary dispersion process and its application as a fluorescent probe in cell labeling. Background technique [0002] Due to their unique semiconductor properties and good biocompatibility, nanodiamonds are widely used as matrix materials for biosensors. Its unique chemical composition and electrical properties make nanodiamonds more conducive to the development of bioelectronics and biosensors than other traditional materials such as gold, silicon, and glass. Most biomedical applications require efficient and reliable diamond surface functions Because biomolecules are generally composed of amino acid sequences, many researchers have noticed that amino-functionalized nanodiamonds have an ideal prospect for biosensors. [0003] In the prior art, there have been re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439B01J13/0008C01P2004/04C01P2004/60C01B32/28G01N33/582G01N33/587C01P2002/72C01P2004/61C01P2004/62C01P2004/64C01P2006/90
Inventor 曹郁方明新蔡小娟史淑瑞刘翠
Owner SHANGHAI BRIGHT STAR BIO-PHARM CO LTD
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