Bio-organic fertilizer and application thereof
A technology of bio-organic fertilizer and compound bacterial agent, applied in the field of bio-organic fertilizer, can solve the problems that soil cannot be comprehensively treated and improved, cannot be propagated well, and crops are difficult to absorb and utilize, etc., so as to improve the utilization rate and effect of fertilizers. Stabilizing, reducing moss effect
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[0025] The preparation method of composite bacterial agent described in the present invention preferably comprises the following steps:
[0026] 1) Lactobacillus rhamnosus, Bacillus subtilis, yeast and photosynthetic bacteria were respectively inoculated in the culture medium for anaerobic fermentation to obtain four bacterial liquids;
[0027] 2) Mixing the four bacterial liquids to obtain a composite microbial agent.
[0028] In the present invention, the preparation process of the composite bacterial agent is carried out under aseptic conditions, and the aseptic room needs to be sterilized before operation; the scope of the sterilizing includes instruments, utensils, clothes and hands.
[0029] In the present invention, Lactobacillus rhamnosus, Bacillus subtilis, saccharomyces and photosynthetic bacteria are activated respectively to obtain activated bacterial liquid; the process and parameters of the activation process are not particularly limited in the present invention,...
Embodiment 1
[0050] Purchase the original strains (Bacillus subtilis, Lactobacillus rhamnosus, yeast and photosynthetic bacteria), activate the strains, and carry out anaerobic fermentation culture.
[0051] Specific operation of anaerobic fermentation culture: medium (1500 parts of purified water, 30 parts of brown sugar, 2 parts of peptone, 4 parts of glucose, 2 parts of yeast extract) sterilization (add 1500L of pure water to a 2000L tank, and when the temperature rises above 50℃ Add the culture medium of the fermenter into the tank under the condition of stirring, after fully stirring evenly, start to sterilize, steam heating to 118°C for 30 minutes to sterilize, after the sterilization, take a sample from the sampling port to test the pH value of the medium. Adjust the value below 6.0, inoculate in the sterile area (use water circulation to cool down, when the tank temperature drops to 35°C, use the flame inoculation method to inoculate), anaerobic fermentation (cultivation temperature...
Embodiment 2
[0059] Weigh 100kg of pepper cake, 5kg of cornmeal, 5kg of bran, 50kg of meat and bone meal, 5kg of medical stone, 25kg of rice bran, 60kg of humic acid, and 100kg of the composite bacterial agent described in Example 1. Mix to obtain bio-organic fertilizer.
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