Unlock instant, AI-driven research and patent intelligence for your innovation.

Oligodendrocyte progenitor cell compositions

An oligodendrocyte and composition technology, which is applied in the field of cell biology and can solve the problems of complex, expensive, non-scalable custom reagents, etc.

Pending Publication Date: 2019-01-15
ASTERIAS BIOTHERAPEUTICS INC
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although efforts have been made to generate OPC populations with low levels of undesired cell types, strategies such as cell sorting and antibody depletion are complex and often require the generation of custom reagents that are expensive and not scalable

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oligodendrocyte progenitor cell compositions
  • Oligodendrocyte progenitor cell compositions
  • Oligodendrocyte progenitor cell compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0127] One aspect of the present disclosure includes a container comprising a composition, wherein the composition comprises a population of cells comprising a plurality of oligodendrocyte progenitor cells (OPCs), and wherein the population of cells comprises less than 15% of the undesired cell type. In one aspect, undesired cell types include cells of epithelial lineage. In one aspect, cells of epithelial lineage are characterized by the presence of one or more markers selected from the group consisting of K7 and PCK. In one aspect, the population of cells comprises less than 2% K7 positive cells. In one aspect, the population of cells comprises less than 0.2% K7 positive cells. In certain aspects, the population of cells comprises less than 5% K7 positive cells. In one aspect, populations of cells share a common genetic background. In one aspect, the population of cells has not undergone cell enrichment. In one aspect, the container is configured for cryopreservation. ...

Embodiment 1

[0138] Example 1 - Method for preparing OPC populations including pretreatment

[0139] Undifferentiated human embryonic stem cells (uhESC) from the H1 line (WA01; Thomson JA, Itskovitz-EldorJ, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. Embryonic stem cell lines derived from human blastocysts (Embryonic stem cell lines derived from human blastocysts). S Science. 1998 Nov 6; 282(5391): 1145-7) in KO-DMEM diluted 1:30 GFR with X-VIVO 10 supplemented with 80ng / mL bFGF (ThermoFisher, PHG0263) and 0.5ng / mL TGF-β1 (R&D System, cat# 240-B) cultured on culture medium. Daily medium changes were complete starting 2 days after subculturing the cells. Undifferentiated hESCs were subcultured using collagenase and manual scraping.

[0140] Expose uhESCs to a cocktail of small molecules to boost cells prior to induction of differentiation. In subculture passages before initiation of differentiation, cells were incubated at approximately 0.8-1.0 × 10 5 cells / cm 2 Inoc...

Embodiment 2

[0144] Example 2 - Comparison of Alternative Methods for Preparing OPC Populations Including Pretreatment

[0145] Undifferentiated human embryonic stem cells (uhESC) from the H1 line (WA01; Thomson JA, Itskovitz-EldorJ, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. Embryonic stem cell lines derived from human blastocysts (Embryonic stem cell lines derived from human blastocysts). Science. 1998 Nov 6; 282(5391): 1145-7) on one of the following matrices: 1:30 dilution in KO-DMEM GFR, recombinant laminin 521 (Corning self-coat cat. no. 354221 or PureCoat), or vitronectin (StemCell Technologies, cat. no. 07180) in cell adhesion dilution buffer (StemCell Technologies, cat. No. 07183) with X- VIVO 10 Medium. Daily medium changes were complete starting 2 days after subculturing the cells. Use collagenase and manual scraping or other non-enzymatic means such as 0.5 mM EDTA in PBS (Life Technologies, cat. no. 15575-020) or ReLeSR TM (Stem Cell Technologies, Cat. No...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Compositions comprising a population of oligodendrocyte progenitor cells (OPCs), as well as methods of making and using the same, are provided. In one aspect, a container comprising a composition, where the composition comprises a population of cells comprising a plurality of OPCs, and where the population of cells comprises less than 15% undesirable cell types is provided. In another aspect, thepopulation of cells comprises less than 15% undesirable epithelial lineage cells. In yet another aspect, the population of cells comprises less than 2% K7 positive cells. In an aspect, a population ofcells comprising a plurality of oligodendrocyte progenitor cells is capable of forming less than one epithelial cyst per 100,000 cells in a cyst assay is provided. An even further aspect of the present disclosure is a container comprising a composition, where the composition comprising a plurality of oligodendrocyte progenitor cells is useful in treating treat stroke, spinal cord injury, and multiple sclerosis.

Description

[0001] Cross References to Related Applications [0002] This application claims priority on the filing date of U.S. Provisional Patent Application Serial No. 62 / 315,454, entitled "Oligodendrocyte Progenitor Cell Compositions," filed March 30, 2016, the contents of which are incorporated herein by reference in their entirety. technical field [0003] The present disclosure relates to the field of cell biology of oligodendrocyte progenitor cells. More specifically, the present disclosure relates to compositions comprising oligodendrocyte progenitor cells, and methods of making and using the same. Background technique [0004] When culturing pluripotent cells, including human embryonic stem cells (hESCs), various degrees of spontaneous differentiation can occur during their expansion. This spontaneous differentiation can subsequently impair the ability of hESCs to undergo directed differentiation into specific cell types, including oligodendrocyte progenitor cells (OPCs). Fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/00C12N5/074C12N5/079
CPCC12N5/0622A61K35/30A61P25/00A61P21/00A61P43/00A61P13/10A61P1/00A01K2207/12C12N2501/11C12N2501/115C12N2501/15C12N2501/385C12N2501/999C12N2506/02A01K2227/10A61P1/04
Inventor E·M·惠特利U·舒卡特-木塔兹R·斯里瓦斯塔瓦N·C·曼利C·C·凯斯
Owner ASTERIAS BIOTHERAPEUTICS INC